GDF3, a BMP inhibitor, regulates cell fate in stem cells and early embryos

被引:127
作者
Levine, AJ [1 ]
Brivanlou, AH [1 ]
机构
[1] Rockefeller Univ, Lab Mol Vertebrate Embryol, New York, NY 10021 USA
来源
DEVELOPMENT | 2006年 / 133卷 / 02期
关键词
GDF3; TGF beta; BMP; embryonic stem cell; inhibitor; Xenopus; human; mouse;
D O I
10.1242/dev.02192
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The TGF beta superfamily of ligands plays key functions in development and disease. in both human and mouse embryonic stem cells, a member of this family, GDF3, is specifically expressed in the pluripotent state. We show that GDF3 is an inhibitor of its own subfamily, blocks classic BMP signaling in multiple contexts, interacts with BMP proteins and is expressed specifically in the node during gastrulation in a pattern consistent with BMP inhibition. Furthermore, we use gain- and reduction-of-function to show that in a species-specific manner, GDF3 regulates both of the two major characteristics of embryonic stem cells: the ability to maintain the undifferentiated state and the ability to differentiate into the full spectrum of cell types.
引用
收藏
页码:209 / 216
页数:8
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