Multichannel microchip electrospray mass spectrometry

被引：340

作者：

Xue, QF

Foret, F

Dunayevskiy, YM

Zavracky, PM

McGruer, NE

Karger, BL

机构：

[1] NORTHEASTERN UNIV,BARNETT INST,BOSTON,MA 02115

[2] NORTHEASTERN UNIV,DEPT CHEM,BOSTON,MA 02115

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 03期

关键词：

D O I：

10.1021/ac9607119

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-RIS), The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel, With the sampling orifice of the MS grounded, it was found that a liquid flow of 100-200 nL/min was necessary to maintain a stable electrospray., The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 x 10(-8) M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel multiple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.

引用

收藏

页码：426 / 430

页数：5

相关论文

共 14 条

[1] Microfabricated structures for integrated DNA analysis [J].

Burns, MA ;

Mastrangelo, CH ;

Sammarco, TS ;

Man, FP ;

Webster, JR ;

Johnson, BN ;

Foerster, B ;

Jones, D ;

Fields, Y ;

Kaiser, AR ;

Burke, DT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5556-5561

[2] A MICROSCALE ELECTROSPRAY INTERFACE FOR ONLINE, CAPILLARY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY OF COMPLEX PEPTIDE MIXTURES [J].

DAVIS, MT ;

STAHL, DC ;

HEFTA, SA ;

LEE, TD .

ANALYTICAL CHEMISTRY, 1995, 67 (24) :4549-4556

[3] MICRO-ELECTROSPRAY MASS-SPECTROMETRY - ULTRA-HIGH-SENSITIVITY ANALYSIS OF PEPTIDES AND PROTEINS [J].

EMMETT, MR ;

CAPRIOLI, RM .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (07) :605-613

[4] ONLINE TIME-OF-FLIGHT MASS-SPECTROMETRIC ANALYSIS OF PEPTIDES SEPARATED BY CAPILLARY ELECTROPHORESIS [J].

FANG, LL ;

ZHANG, R ;

WILLIAMS, ER ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1994, 66 (21) :3696-3701

[5] OPEN-CHANNEL ELECTROCHROMATOGRAPHY ON A MICROCHIP [J].

JACOBSON, SC ;

HERGENRODER, R ;

KOUTNY, LB ;

RAMSEY, JM .

ANALYTICAL CHEMISTRY, 1994, 66 (14) :2369-2373

[6] EFFECTS OF INJECTION SCHEMES AND COLUMN GEOMETRY ON THE PERFORMANCE OF MICROCHIP ELECTROPHORESIS DEVICES [J].

JACOBSON, SC ;

HERGENRODER, R ;

KOUTNY, LB ;

WARMACK, RJ ;

RAMSEY, JM .

ANALYTICAL CHEMISTRY, 1994, 66 (07) :1107-1113

[7]

Ko W.H, 1989, SENSORS COMPREHENSIV, V1, P107

[8] PLANAR CHIPS TECHNOLOGY FOR MINIATURIZATION AND INTEGRATION OF SEPARATION TECHNIQUES INTO MONITORING SYSTEMS - CAPILLARY ELECTROPHORESIS ON A CHIP [J].

MANZ, A ;

HARRISON, DJ ;

VERPOORTE, EMJ ;

FETTINGER, JC ;

PAULUS, A ;

LUDI, H ;

WIDMER, HM .

JOURNAL OF CHROMATOGRAPHY, 1992, 593 (1-2) :253-258

[9] Chip PCR .1. Surface passivation of microfabricated silicon-glass chips for PCR [J].

Shoffner, MA ;

Cheng, J ;

Hvichia, GE ;

Kricka, LJ ;

Wilding, P .

NUCLEIC ACIDS RESEARCH, 1996, 24 (02) :375-379

[10] ATTOMOLE-SENSITIVITY ELECTROSPRAY SOURCE FOR LARGE-MOLECULE MASS-SPECTROMETRY [J].

VALASKOVIC, GA ;

KELLEHER, NL ;

LITTLE, DP ;

AASERUD, DJ ;

MCLAFFERTY, FW .

ANALYTICAL CHEMISTRY, 1995, 67 (20) :3802-3805