ATTOMOLE-SENSITIVITY ELECTROSPRAY SOURCE FOR LARGE-MOLECULE MASS-SPECTROMETRY

被引：199

作者：

VALASKOVIC, GA ^{[1
]}

KELLEHER, NL ^{[1
]}

LITTLE, DP ^{[1
]}

AASERUD, DJ ^{[1
]}

MCLAFFERTY, FW ^{[1
]}

机构：

[1] CORNELL UNIV,BAKER LAB CHEM,ITHACA,NY 14853

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 20期

关键词：

D O I：

10.1021/ac00116a030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (>10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 mu m i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of >10(5) for full mass spectra, while the total amount of material consumed is similar to 150 and 10 amol, respectively.

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页码：3802 / 3805

页数：4

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