4-hydroxynonenal interaction with rhodopsin

被引：5

作者：

vanKuijk, FJGM

机构：

[1] Dept. of Ophthalmol. and Vis. Sci., 7-138 Medical Research Building, University of Texas Medical Branch, Galveston

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 230卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.5942

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

4-Hydroxynonenal binds easily to rhodopsin and this was accompanied by a decrease in measurable sulfhydryl groups. Analysis of tryptic digests of the rhodopsin-HNE adduct by high performance liquid chromatography revealed that several peptides present in the digests of rhodopsin disappeared, whereas HNE modified peptides not originally present were found in digests of the rhodopsin-HNE adduct. Matrix assisted laser desorption time of flight mass spectrometry showed that up to ten molecules of HNE bound to rhodopsin. (C) 1997 Academic Press

引用

页码：275 / 279

页数：5

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