Reactive oxygen species-related plasma effects on the apoptosis of human bladder cancer cells in atmospheric pressure pulsed plasma jets

被引：81

作者：

Joh, Hea Min ^{[1
]}

Kim, Sun Ja ^{[1
]}

Chung, T. H. ^{[1
]}

Leem, S. H. ^{[2
]}

机构：

[1] Dong A Univ, Dept Phys, Pusan 604714, South Korea

[2] Dong A Univ, Dept Biol Sci, Pusan 604714, South Korea

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

MEDICINE;

D O I：

10.1063/1.4742742

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Helium/oxygen atmospheric pressure plasma jets driven by pulsed dc voltage with repetition rate of several tens of kilohertz are utilized for plasma-cell interactions. The effect of operating parameters on the apoptosis of cultured human bladder (EJ) cancer cells is investigated. The parameters such as applied voltage, pulse repetition frequency, and duty ratio determine the plasma dose. The apoptotic changes in cells with plasma treatment are detected by staining assay and flow cytometry. Apoptosis rates are observed to correlate well with both the plasma dose and the levels of intracellular and extracellular reactive oxygen species. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4742742]

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页数：5

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