DNA damage and mitochondria dysfunction in cell apoptosis induced by nonthermal air plasma

被引：198

作者：

Kim, G. J. ^{[2
]}

Kim, W. ^{[1
]}

Kim, K. T. ^{[1
,3
]}

Lee, J. K. ^{[2
]}

机构：

[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Dept Life Sci, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Dept Elect & Elect Engn, Pohang 790784, South Korea

[3] Pohang Univ Sci & Technol, Dept Integrat Biosci & Bioengn, Pohang 790784, South Korea

来源：

APPLIED PHYSICS LETTERS | 2010年 / 96卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

biochemistry; biological effects of radiation; biomembranes; cancer; cellular biophysics; DNA; molecular biophysics; plasma applications; radiation therapy; tumours;

D O I：

10.1063/1.3292206

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nonthermal plasma is known to induce animal cell death but the mechanism is not yet clear. Here, cellular and biochemical regulation of cell apoptosis is demonstrated for plasma treated cells. Surface type nonthermal air plasma triggered apoptosis of B16F10 mouse melanoma cancer cells causing DNA damage and mitochondria dysfunction. Plasma treatment activated caspase-3, apoptosis executioner. The plasma treated cells also accumulated gamma-H2A.X, marker for DNA double strand breaks, and p53 tumor suppressor gene as a response to DNA damage. Interestingly, cytochrome C was released from mitochondria and its membrane potential was changed significantly.

引用

页数：3

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