How Ubiquitin Unfolds after Transfer into the Gas Phase

被引：95

作者：

Skinner, Owen S. ^{[3
]}

McLafferty, Fred W. ^{[3
]}

Breuker, Kathrin ^{[1
,2
]}

机构：

[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

[2] Univ Innsbruck, Ctr Mol Biosci Innsbruck, A-6020 Innsbruck, Austria

[3] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2012年 / 23卷 / 06期

基金：

奥地利科学基金会; 美国国家卫生研究院;

关键词：

Electron capture dissociation; Protein; Ubiquitin; Gas phase structure; ELECTRON-CAPTURE DISSOCIATION; NATIVE CYTOCHROME-C; MASS-SPECTROMETRY; NONCOVALENT INTERACTIONS; PROTEIN CATIONS; LIGAND-BINDING; TOP-DOWN; IONS; TRANSITIONS; ENVIRONMENT;

D O I：

10.1007/s13361-012-0370-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural evolution of ubiquitin after transfer into the gas phase was studied by electron capture dissociation. Site-specific fragment yields show that ubiquitin's solution fold is overall unstable in the gas phase, but unfolding caused by loss of solvent is slowest in regions stabilized by salt bridges.

引用

页码：1011 / 1014

页数：4

共 22 条

[1] The methanol-induced conformational transitions of β-lactoglobulin, cytochrome c, and ubiquitin at low pH:: A study by electrospray ionization mass spectrometry [J].

Babu, KR ;

Moradian, A ;

Douglas, DJ .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2001, 12 (03) :317-328

[2] Evidence for unfolding and refolding of gas-phase cytochrome c ions in a Paul trap [J].

Badman, ER ;

Myung, S ;

Clemmer, DE .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2005, 16 (09) :1493-1497

[3] Dissociation of different conformations of ubiquitin ions [J].

Badman, ER ;

Hoaglund-Hyzer, CS ;

Clemmer, DE .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2002, 13 (06) :719-723

[4] The thermal unfolding of native cytochrome c in the transition from solution to gas phase probed by native electron capture dissociation [J].

Breuker, K ;

McLafferty, FW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (31) :4911-4914

[5] Detailed unfolding and folding of gaseous ubiquitin ions characterized by electron capture dissociation [J].

Breuker, K ;

Oh, HB ;

Horn, DM ;

Cerda, BA ;

McLafferty, FW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) :6407-6420

[6] Nonergodic and conformational control of the electron capture dissociation of protein cations [J].

Breuker, K ;

Oh, HB ;

Lin, C ;

Carpenter, BK ;

McLafferty, FW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (39) :14011-14016

[7] Native electron capture dissociation for the structural characterization of noncovalent interactions in native cytochrome c [J].

Breuker, K ;

McLafferty, FW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (40) :4900-4904

[8]

Breuker K., 2006, Principles of Mass Spectrometry Applied to Biomolecules, P177

[9] Electrostatic Stabilization of a Native Protein Structure in the Gas Phase [J].

Breuker, Kathrin ;

Brueschweiler, Sven ;

Tollinger, Martin .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (04) :873-877

[10] Stepwise evolution of protein native structure with electrospray into the gas phase, 10-12 to 102 S [J].

Breuker, Kathrin ;

McLafferty, Fred W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18145-18152

← 1 2 3 →