Ratiometric Biosensor for Aggregation-Induced Emission-Guided Precise Photodynamic Therapy

被引：265

作者：

Han, Kai ^{[1
,2
]}

Wang, Shi-Bo ^{[1
,2
,3
]}

Lei, Qi ^{[1
,2
]}

Zhu, Jing-Yi ^{[1
,2
]}

Zhang, Xian-Zheng ^{[1
,2
,3
]}

机构：

[1] Wuhan Univ, Minist Educ, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[3] Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China

来源：

ACS NANO | 2015年 / 9卷 / 10期

基金：

中国国家自然科学基金;

关键词：

photodynamic therapy; ratiometric biosensor; MMP-2; responsive; aggregation-induced emission; tumor; IN-VIVO; FLUORESCENT-PROBE; CHIMERIC PEPTIDE; DELIVERY; NANOPARTICLES; APOPTOSIS; SENSOR; TUMORS; DRUGS;

D O I：

10.1021/acsnano.5b04243

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Photodynamic therapy faces the barrier of choosing the appropriate irradiation region and time. In this paper, a matrix metalloproteinase-2 (MMP-2) responsive ratiometric biosensor was designed and synthesized for aggregation-induced emission (AIE)-guided precise photodynamic therapy. It was found that the biosensor presented the MMP-2 responsive AIE behavior. Most importantly, it could accurately differentiate the tumor cells from the healthy cells by the fluorescence ratio between freed tetraphenylethylene and protoporphyrin IX (PpIX, internal reference). In vivo study demonstrated that the biosensor could preferentially accumulate in the tumor tissue with a relative long blood retention time. Note that the intrinsic fluorescence of PpIX and MMP-2-triggered AIE fluorescence provided a real-time feedback which guided precise photodynamic therapy in vivo efficiently. This strategy demonstrated here opens a window in the precise medicine, especially for phototherapy.

引用

页码：10268 / 10277

页数：10

共 36 条

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Detection of MMP-2 and MMP-9 Activity in Vivo with a Triple-Helical Peptide Optical Probe [J].