Fabrication of amperometric xanthine biosensors based on direct chemistry of xanthine oxidase

被引：34

作者：

Gao, Yansheng ^{[1
]}

Shen, Chunping ^{[1
]}

Di, Junwei ^{[1
]}

Tu, Yifeng ^{[1
]}

机构：

[1] Soochow Univ, Dept Chem, Suzhou 215123, Jiangsu, Peoples R China

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2009年 / 29卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Xanthine; Biosensor; Xanthine oxidase; Multi-wall carbon nanotubes; Sol-gel; DIRECT ELECTRON-TRANSFER; GLASSY-CARBON ELECTRODE; FISH FRESHNESS; DIRECT ELECTROCHEMISTRY; SUPEROXIDE-DISMUTASE; GOLD ELECTRODE; NANOTUBES; NANOPARTICLES; CONSTRUCTION; HYPOXANTHINE;

D O I：

10.1016/j.msec.2009.05.004

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

082905 [生物质能源与材料]; 100103 [病原生物学];

摘要：

The construction of amperometric xanthine biosensor by immobilization of xanthine oxidase (XOD) on the multi-wall carbon nanotubes (CNTs) modified glassy carbon (GC) electrode surface was investigated. The direct chemistry of XOD was accomplished and the formal potential was about - 0.465 V (vs SCE). The heterogeneous electron transfer rate constant was evaluated to be 2.0 +/- 0.3 s(-1). The xanthine biosensor based on XOD entrapped in silica sol-gel (SG) thin film on CNTs-modified GC electrode surface was also investigated. The XOD still maintains its activity to xanthine. The amperometric response to xanthine showed a linear relation in the range from 0.2 mu M to 10 mu M and a detection limit of 0.1 mu M (S/N = 3). The enzyme electrode retained 95% of its initial activity after 90 days of storage. The sensor exhibited high sensitivity, rapid response and good long-term stability. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2213 / 2216

页数：4

共 27 条

[1]

Amperometric biosensor for hypoxanthine based on immobilized xanthine oxidase on nanocrystal gold-carbon paste electrodes [J].