Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces

被引：58

作者：

de Crécy-Lagard, V ^{[1
]}

Servant-Moisson, P ^{[1
]}

Viala, J ^{[1
]}

Grandvalet, C ^{[1
]}

Mazodier, P ^{[1
]}

机构：

[1] Inst Pasteur, Unite Biochim Microbienne, CNRS, URA 1300, F-75724 Paris, France

来源：

MOLECULAR MICROBIOLOGY | 1999年 / 32卷 / 03期

关键词：

D O I：

10.1046/j.1365-2958.1999.01364.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP-dependent protease family Cip were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2, located in tandem upstream from the clpX gene, and at least two unlinked clpC genes. Disruption of the clpP1 gene in S. lividans and S. coelicolor blocks differentiation at the substrate mycelium step. Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans, S. albus and S. coelicolor. Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans.

引用

页码：505 / 517

页数：13

共 69 条

[1] The proteasome [J].

Baumeister, W ;

Lupas, A .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (02) :273-278

[2] The regulation of antibiotic production in Streptomyces coelicolor A3(2) [J].

Bibb, M .

MICROBIOLOGY-SGM, 1996, 142 :1335-1344

[3] Antibiotic resistance gene cassettes derived from the Omega interposon for use in E-coli and Streptomyces [J].

BlondeletRouault, MH ;

Weiser, J ;

Lebrihi, A ;

Branny, P ;

Pernodet, JL .

GENE, 1997, 190 (02) :315-317

[4] COMPUTER-ASSISTED IDENTIFICATION AND CLASSIFICATION OF STREPTOMYCETE PROMOTERS [J].

BOURN, WR ;

BABB, B .

NUCLEIC ACIDS RESEARCH, 1995, 23 (18) :3696-3703

[5] Production of actinorhodin-related ''blue pigments'' by Streptomyces coelicolor A3(2) [J].

Bystrykh, LV ;

FernandezMoreno, MA ;

Herrema, JK ;

Malpartida, F ;

Hopwood, DA ;

Dijkhuizen, L .

JOURNAL OF BACTERIOLOGY, 1996, 178 (08) :2238-2244

[6]

CHAMPNESS WC, 1994, REGULATION OF BACTERIAL DIFFERENTIATION, P61

[7] GENETICS OF DIFFERENTIATION IN STREPTOMYCES [J].

CHATER, KF .

ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 :685-713

[8]

CHATER KF, 1980, J GEN MICROBIOL, V116, P323

[9]

CHATER KF, 1997, BACTERIA MULTICELLUL, P149

[10] The cyanobacterium Synechococcus sp PCC 7942 possesses a close homologue to the chloroplast ClpC protein of higher plants [J].

Clarke, AK ;

Eriksson, MJ .

PLANT MOLECULAR BIOLOGY, 1996, 31 (04) :721-730

← 1 2 3 4 5 6 7 →