Identification of novel retromer complexes in the mouse testis

被引：15

作者：

Kim, Ekyune ^{[1
]}

Lee, Jae-Woong ^{[1
]}

Baek, Dong-Chul ^{[1
,2
]}

Lee, Sang-Rae ^{[1
]}

Kim, Myeong-Su ^{[1
]}

Kim, Sang-Hyun ^{[1
]}

Imakawa, Kazuhiko ^{[3
]}

Chang, Kyu-Tae ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Natl Primate Res Center, Ochang Eup 363883, Chung Buk, South Korea

[2] Jeju Natl Univ, Fac Biotechnol, Jejusi 690756, Jeju, South Korea

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 01期

关键词：

retromer complex; vacuolar protein sorting; embryonic development; endosome; spermatogenesis;

D O I：

10.1016/j.bbrc.2008.07.067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay Using antibodies raised against each Vps component. Our results revealed that the retromer complex Could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：16 / 21

页数：6

共 20 条

[1] Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor [J].

Arighi, CN ;

Hartnell, LM ;

Aguilar, RC ;

Haft, CR ;

Bonifacino, JS .

JOURNAL OF CELL BIOLOGY, 2004, 165 (01) :123-133

[2] Sorting nexin-1 mediates tubular endosome-to-TGN transport through coincidence sensing of high-curvature membranes and 3-phosphoinositides [J].

Carlton, J ;

Bujny, M ;

Peter, BJ ;

Oorschot, VMJ ;

Rutherford, A ;

Mellor, H ;

Klumperman, J ;

McMahon, HT ;

Cullen, PJ .

CURRENT BIOLOGY, 2004, 14 (20) :1791-1800

[3] Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly [J].

Collins, BM ;

Skinner, CF ;

Watson, PJ ;

Seaman, MNJ ;

Owen, DJ .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (07) :594-602

[4] The human Vps29 retromer component is a metallo-phosphoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide [J].

Damen, Ester ;

Krieger, Elmar ;

Nielsen, Jens E. ;

Eygensteyn, Jelle ;

van Leeuwen, Jeroen E. M. .

BIOCHEMICAL JOURNAL, 2006, 398 (399-409) :399-409

[5] The subapical compartment: a traffic center in membrane polarity development [J].

Hoekstra, D ;

Tyteca, D ;

van IJzendoorn, SCD .

JOURNAL OF CELL SCIENCE, 2004, 117 (11) :2183-2192

[6] Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells [J].

Kashiwabara, S ;

Zhuang, TG ;

Yamagata, K ;

Noguchi, J ;

Fukamizu, A ;

Baba, T .

DEVELOPMENTAL BIOLOGY, 2000, 228 (01) :106-115

[7] A novel mammalian retromer component, Vps26B [J].

Kerr, MC ;

Bennetts, JS ;

Simpson, F ;

Thomas, EC ;

Flegg, C ;

Gleeson, PA ;

Wicking, C ;

Teasdale, RD .

TRAFFIC, 2005, 6 (11) :991-1001

[8] Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain [J].

Kim, BY ;

Ueda, M ;

Nakamura, Y ;

Kohsaka, S ;

Akazawa, C .

NEUROSCIENCE LETTERS, 2004, 355 (03) :217-220

[9] Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm [J].

Kim, E ;

Nishimura, H ;

Baba, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (02) :313-319

[10]

LEE JJ, 1992, DEVELOPMENT, V115, P277

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