Environmental genomics reveals a single-species ecosystem deep within earth

被引：323

作者：

Chivian, Dylan ^{[1
,2
]}

Brodie, Eoin L. ^{[2
,3
]}

Alm, Eric J. ^{[2
,4
,5
]}

Culley, David E. ^{[6
]}

Dehal, Paramvir S. ^{[1
,2
]}

DeSantis, Todd Z. ^{[2
,3
]}

Gihring, Thomas M. ^{[7
]}

Lapidus, Alla ^{[8
]}

Lin, Li-Hung ^{[9
]}

Lowry, Stephen R. ^{[8
]}

Moser, Duane P. ^{[10
]}

Richardson, Paul M. ^{[8
]}

Southam, Gordon ^{[11
]}

Wanger, Greg ^{[11
]}

Pratt, Lisa M. ^{[12
,13
]}

Andersen, Gary L. ^{[2
,3
]}

Hazen, Terry C. ^{[2
,3
,13
]}

Brockman, Fred J. ^{[14
]}

Arkin, Adam P. ^{[1
,2
,15
]}

Onstott, Tullis C. ^{[13
,16
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[2] Virtual Inst Microbial Stress & Survival, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Earth Sci, Berkeley, CA 94720 USA

[4] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[5] MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA

[6] Pacific NW Natl Lab, Energy & Efficiency Technol Div, Richland, WA 99352 USA

[7] Florida State Univ, Dept Oceanog, Tallahassee, FL 32306 USA

[8] US DOE, Joint Genom Inst, Genom Technol Program, Berkeley, CA 94598 USA

[9] Natl Taiwan Univ, Dept Geosci, Taipei 106, Taiwan

[10] Desert Res Inst, Div Earth & Ecosyst Sci, Las Vegas, NV 89119 USA

[11] Univ Western Ontario, Dept Earth Sci, London, ON N6A 5B7, Canada

[12] Indiana Univ, Dept Geol Sci, Bloomington, IN 47405 USA

[13] NASA Astrobiol Inst, IPTAI, Bloomington, IN 47405 USA

[14] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[15] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[16] Princeton Univ, Dept Geosci, Princeton, NJ 08544 USA

来源：

SCIENCE | 2008年 / 322卷 / 5899期

关键词：

D O I：

10.1126/science.1155495

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA from low-biodiversity fracture water collected at 2.8-kilometer depth in a South African gold mine was sequenced and assembled into a single, complete genome. This bacterium, Candidatus Desulforudis audaxviator, composes > 99.9% of the microorganisms inhabiting the fluid phase of this particular fracture. Its genome indicates a motile, sporulating, sulfate-reducing, chemoautotrophic thermophile that can fix its own nitrogen and carbon by using machinery shared with archaea. Candidatus Desulforudis audaxviator is capable of an independent life-style well suited to long-term isolation from the photosphere deep within Earth's crust and offers an example of a natural ecosystem that appears to have its biological component entirely encoded within a single genome.

引用

页码：275 / 278

页数：4

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