DNA-based biosensor platforms for the detection of TP53 mutation

被引:33
作者
Altintas, Z. [1 ,2 ]
Tothill, I. E. [1 ]
机构
[1] Cranfield Univ, Cranfield Hlth, Cranfield MK43 0AL, Beds, England
[2] Sabanci Univ, Fac Nat Sci & Engn, TR-34956 Istanbul, Turkey
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 169卷
关键词
Surface plasmon resonance; SPR; Quartz crystal microbalance; QCM; DNA mutation; TP53; Cancer; SURFACE; MISMATCHES;
D O I
10.1016/j.snb.2012.04.064
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A DNA-based assay for the detection of one-point mutation in TP53 gene, responsible for lung cancer, was developed using a surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM) biosensor systems. Amine coupling was employed for the immobilization of NeutrAvidin on thiol-derivatized surface to capture the biotinylated target sequence. Two targets sequences and one control DNA sequence were investigated including, a fully complementary (30 mer), one-point mutation and a non-complimentary DNA using hybridization with a detection probe strand (27 met). The most appropriate surface coating was also examined for both sensor platforms with hybridization and single nucleotide polymorphism (SNP) detection efficiency were then compare. A 0.03-2 mu M concentration range of detection probe was detected using the SPR and QCM sensors on wild and mutant type target surfaces. The linear regression analysis was performed for both sensors resulting in a R-2 value for the SPR assay of 0.985 and 0.993 for perfect and mismatch reaction and of 0.978 and 0.976 for the QCM assay, respectively. The obtained results demonstrate that the used approach represents a very promising future method for the detection of one-point mutation in genetic-based health problem with highly sensitive, specific, and real-time analysis. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:188 / 194
页数:7
相关论文
共 30 条
[11]   Detection of TP53 mutation using a portable surface plasmon resonance DNA-based biosensor [J].
Jiang, TS ;
Minunni, M ;
Wilson, P ;
Zhang, J ;
Turner, APF ;
Mascini, M .
BIOSENSORS & BIOELECTRONICS, 2005, 20 (10) :1939-1945
[12]   Hairpin DNA probe based electrochemical biosensor using methylene blue as hybridization indicator [J].
Jin, Yan ;
Yao, Xin ;
Liu, Qing ;
Li, Jinghong .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (06) :1126-1130
[13]  
Montgomery K. T., 2008, CURRENT PROTOCOLS HU, V7, P16
[14]   Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance [J].
Nakatani, K ;
Sando, S ;
Saito, I .
NATURE BIOTECHNOLOGY, 2001, 19 (01) :51-55
[15]   Endogenous Control Genes in Prostate Cells: Evaluation of Gene Expression Using 'Real-Time' Quantitative Polymerase Chain Reaction [J].
Nna, Emmanuel ;
Tothill, Ibtisam E. ;
Ludeman, Linmarie ;
Bailey, Tracey .
MEDICAL PRINCIPLES AND PRACTICE, 2010, 19 (06) :433-439
[16]   Point mutation detection with the sandwich method employing hydrogel nanospheres by the surface plasmon resonance imaging technique [J].
Okumura, A ;
Sato, Y ;
Kyo, M ;
Kawaguchi, H .
ANALYTICAL BIOCHEMISTRY, 2005, 339 (02) :328-337
[17]   The IARC TP53 database: New Online mutation analysis and recommendations to users [J].
Olivier, M ;
Eeles, R ;
Hollstein, M ;
Khan, MA ;
Harris, CC ;
Hainaut, P .
HUMAN MUTATION, 2002, 19 (06) :607-614
[18]   Review on carbon-derived, solid-state, micro and nano sensors for electrochemical sensing applications [J].
Qureshi, Anjum ;
Kang, Weng P. ;
Davidson, Jimmy L. ;
Gurbuz, Yasar .
DIAMOND AND RELATED MATERIALS, 2009, 18 (12) :1401-1420
[19]   Surface plasmon resonance imaging on a microchip for detection of DNA-modified gold nanoparticles deposited onto the surface in a non-cross-linking configuration [J].
Sato, Yasunobu ;
Sato, Kae ;
Hosokawa, Kazuo ;
Maeda, Mizuo .
ANALYTICAL BIOCHEMISTRY, 2006, 355 (01) :125-131
[20]   Prevalence of positive selection among nearly neutral amino acid replacements in Drosophila [J].
Sawyer, Stanley A. ;
Parsch, John ;
Zhang, Zhi ;
Hartl, Daniel L. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (16) :6504-6510