Enhancement by α-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-γ through the upregulation of protein kinase C in rat vascular smooth muscle cells

被引：14

作者：

Kogure, K ^{[1
]}

Morita, M ^{[1
]}

Hama, S ^{[1
]}

Nakashima, S ^{[1
]}

Tokumura, A ^{[1
]}

Fukuzawa, K ^{[1
]}

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2002年 / 269卷 / 09期

关键词：

alpha-tocopheryl hemisuccunate; alpha-tocopherol; nitric oxide; vascular smooth muscle cells; protein kinase C;

D O I：

10.1046/j.1432-1033.2002.02894.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of alpha-tocopheryl hemisuccinate (TS) on lipopolysaccharide (LPS)/interferon-gamma (IFN)-induced nitric oxide production in rat vascular smooth muscle cells (VSMC) was examined. The LPS/IFN-induced NO production was enhanced by TS but not by the otheralpha-tocopherol (alpha-T) derivatives alpha-tocopheryl acetate (TA) and alpha-tocopheryl nicotinate (TN), or alpha-T itself. alpha-T, TA and TN inhibited the enhancement by TS of LPS/IFN-induced NO production. The enhancing effect of TS was observed in the presence of LPS, but not IFN, suggesting that TS participates in the LPS-stimulated signal pathway leading to NO production. Protein kinase C (PKC) inhibitors, but not protein kinase A inhibitors, inhibited the enhancing effect of TS on LPS/IFN-induced NO production. Furthermore, TS enhanced the amount of PKCalpha in VSMC. From these results, we concluded that the enhancing effect of LPS/IFN-induced NO production was caused by upregulation of PKC in VSMC.

引用

页码：2367 / 2372

页数：6

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