Measurement of the binding of proteins to polyelectrolytes by frontal analysis continuous capillary electrophoresis

被引：102

作者：

Gao, JY ^{[1
]}

Dubin, PL ^{[1
]}

Muhoberac, BB ^{[1
]}

机构：

[1] INDIANA UNIV PURDUE UNIV,DEPT CHEM,INDIANAPOLIS,IN 46202

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 15期

关键词：

D O I：

10.1021/ac970026h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a novel technique, frontal analysis continuous capillary electrophoresis (FACCE), to study the binding of proteins to polyelectrolytes. Compared with existing electrophoresis methods such as conventional frontal analysis and the Hummel-Dreyer method, FACCE offers enhanced lower detection limits and is free from effects due to slow binding kinetics, thus making it suitable for studying equilibrium systems, In addition, with a single calibration, FACCE provides for efficient quantitative analysis. Here we report results obtained with beta-lactoglobulin as the ligand and sodium poly(styrenesulfonate) (NaPSS) as the ligand-binding substrate. For this model system, FACCE yields reproducible calibration curves and binding isotherms. The binding parameters so determined are compared with previous results for other protein-polyelectrolyte systems.

引用

页码：2945 / 2951

页数：7

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