Measurement of the binding of proteins to polyelectrolytes by frontal analysis continuous capillary electrophoresis

被引:102
作者
Gao, JY [1 ]
Dubin, PL [1 ]
Muhoberac, BB [1 ]
机构
[1] INDIANA UNIV PURDUE UNIV,DEPT CHEM,INDIANAPOLIS,IN 46202
关键词
D O I
10.1021/ac970026h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have developed a novel technique, frontal analysis continuous capillary electrophoresis (FACCE), to study the binding of proteins to polyelectrolytes. Compared with existing electrophoresis methods such as conventional frontal analysis and the Hummel-Dreyer method, FACCE offers enhanced lower detection limits and is free from effects due to slow binding kinetics, thus making it suitable for studying equilibrium systems, In addition, with a single calibration, FACCE provides for efficient quantitative analysis. Here we report results obtained with beta-lactoglobulin as the ligand and sodium poly(styrenesulfonate) (NaPSS) as the ligand-binding substrate. For this model system, FACCE yields reproducible calibration curves and binding isotherms. The binding parameters so determined are compared with previous results for other protein-polyelectrolyte systems.
引用
收藏
页码:2945 / 2951
页数:7
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