Chromatin Modulation at the FLO11 Promoter of Saccharomyces cerevisiae by HDAC and Swi/Snf Complexes

被引:24
作者
Barrales, Ramon R. [1 ]
Korber, Philipp [2 ]
Jimenez, Juan [1 ]
Ibeas, Jose I. [1 ]
机构
[1] Univ Pablo Olavide, CSIC, Ctr Andaluz Biol Desarrollo, Seville 41013, Spain
[2] Univ Munich, Adolf Butenandt Inst, D-80336 Munich, Germany
关键词
RPD3 HISTONE DEACETYLASE; TRANSCRIPTION FACTOR; INVASIVE GROWTH; GENE-EXPRESSION; PROTEIN-KINASE; YEAST; REPRESSION; RECRUITMENT; ACTIVATION; UME6;
D O I
10.1534/genetics.112.140301
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cell adhesion and biofilm formation are critical processes in the pathogenicity of fungi and are mediated through a family of adhesin proteins conserved throughout yeasts and fungi. In Saccharomyces cerevisiae, Flo11 is the main adhesin involved in cell adhesion and biofilm formation, making the study of its function and regulation in this nonpathogenic budding yeast highly relevant. The S. cerevisiae FLO11 gene is driven by a TATA-box-containing promoter that is regulated through one of the longest regulatory upstream regions (3 kb) in yeast. We reported recently that two chromatin cofactor complexes, the Rpd3L deacetylase and the Swi/Snf chromatin-remodeling complexes, contribute significantly to the regulation of FLO11. Here, we analyze directly how these complexes impact on FLO11 promoter chromatin structure and dissect further the interplay between histone deacetylases, chromatin remodeling, and the transcriptional repressor Sfl1. We show that the regulation of chromatin structure represents an important layer of control in the highly complex regulation of the FLO11 promoter.
引用
收藏
页码:791 / U220
页数:16
相关论文
共 51 条
[41]   Histone deacetylases RPD3 and HOS2 regulate the transcriptional activation of DNA damage-inducible genes [J].
Sharma, Vishva Mitra ;
Tomar, Raghuvir S. ;
Dempsey, Alison E. ;
Reese, Joseph C. .
MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (08) :3199-3210
[42]   Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment [J].
Shevchenko, Anna ;
Roguev, Assen ;
Schaft, Daniel ;
Buchanan, Luke ;
Habermann, Bianca ;
Sakalar, Cagri ;
Thomas, Henrik ;
Krogan, Nevan J. ;
Shevchenko, Andrej ;
Stewart, A. Francis .
GENOME BIOLOGY, 2008, 9 (11)
[43]   SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast [J].
StrahlBolsinger, S ;
Hecht, A ;
Luo, KH ;
Grunstein, M .
GENES & DEVELOPMENT, 1997, 11 (01) :83-93
[44]   INTERPLAY BETWEEN NUCLEOSOMES AND TRANSCRIPTION FACTORS AT THE YEAST PHO5 PROMOTER [J].
SVAREN, J ;
HORZ, W .
SEMINARS IN CELL BIOLOGY, 1995, 6 (04) :177-183
[45]   Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae [J].
van Dyk, D ;
Pretorius, IS ;
Bauer, FF .
GENETICS, 2005, 169 (01) :91-106
[46]   A canonical promoter organization of the transcription machinery and its regulators in the Saccharomyces genome [J].
Venters, Bryan J. ;
Pugh, B. Franklin .
GENOME RESEARCH, 2009, 19 (03) :360-371
[47]   Flocculation, adhesion and biofilm formation in yeasts [J].
Verstrepen, KJ ;
Klis, FM .
MOLECULAR MICROBIOLOGY, 2006, 60 (01) :5-15
[48]   Origins of variation in the fungal cell surface [J].
Verstrepen, KJ ;
Reynolds, TB ;
Fink, GR .
NATURE REVIEWS MICROBIOLOGY, 2004, 2 (07) :533-540
[49]   RPD3 ENCODES A 2ND FACTOR REQUIRED TO ACHIEVE MAXIMUM POSITIVE AND NEGATIVE TRANSCRIPTIONAL STATES IN SACCHAROMYCES-CEREVISIAE [J].
VIDAL, M ;
GABER, RF .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (12) :6317-6327
[50]   Requirement of Hos2 histone deacetylase for gene activity in yeast [J].
Wang, A ;
Kurdistani, SK ;
Grunstein, M .
SCIENCE, 2002, 298 (5597) :1412-1414