SPR-based immunosensor for determining staphylococcal enterotoxin A

被引:61
作者
Tsai, Wen-Chi [1 ]
Li, Ie-Chin [1 ]
机构
[1] Chinese Culture Univ, Grad Inst Biotechnol, Taipei, Taiwan
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 136卷 / 01期
关键词
Surface plasmon resonance; Mixed self-assembled monolayers; Staphylococcal enterotoxin A; Immunosensor; SURFACE-PLASMON RESONANCE; SELF-ASSEMBLED MONOLAYER; PIEZOELECTRIC CRYSTAL IMMUNOSENSOR; LINKED-IMMUNOSORBENT-ASSAY; ENHANCED PERFORMANCE; BIOSENSOR;
D O I
10.1016/j.snb.2008.10.061
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An immunosensor for determining staphylococcal enterotoxin A (SEA) was developed on the basis of surface plasmon resonance (SPR). The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 16-mercapto-1-hexanol (16-MHA) to 6-mercapto-1-hexanol (6-MCH). The surface functionalized with a 1:20 mixed self-assembled monolayer(SAM) exhibited the best result in terms of SEA detection sensitivity. Through optimization of anti-SEA immobilization, the SPR sensor could detect SEA in phosphate-buffered saline buffer in a linear range from 100 to 1000ng/ml. Furthermore, studies of recovery and matrix effects were performed to evaluate the suitability of the developed immunosensor for directly analyzing SEA in milk samples. Results indicated that the mixed SAM-based SPR immunosensor has the potential for the specific detection of SEA. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:8 / 12
页数:5
相关论文
共 31 条
[1]  
ARCHER D L, 1988, Clinical Microbiology Reviews, V1, P377
[2]   An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk [J].
Asao, T ;
Kumeda, Y ;
Kawai, T ;
Shibata, T ;
Oda, H ;
Haruki, K ;
Nakazawa, H ;
Kozaki, S .
EPIDEMIOLOGY AND INFECTION, 2003, 130 (01) :33-40
[3]   Staphylococcal enterotoxins [J].
Balaban, N ;
Rasooly, A .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2000, 61 (01) :1-10
[4]  
BERGER M, 1967, BIBL GYNAECOL, P1
[5]   HIGHLY SENSITIVE FLUOROGENIC ENZYME-LINKED-IMMUNOSORBENT-ASSAY - DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN-B [J].
BHATTI, AR ;
SIDDIQUI, YM ;
MICUSAN, VV .
JOURNAL OF MICROBIOLOGICAL METHODS, 1994, 19 (03) :179-187
[6]   Comparison of random and oriented immobilisation of antibody fragments on mixed self-assembled monolayers [J].
Bonroy, Kristien ;
Frederix, Filip ;
Reekmans, Gunter ;
Dewolf, Ellen ;
De Palma, Randy ;
Borghs, Gustaaf ;
Declerck, Paul ;
Goddeeris, Bruno .
JOURNAL OF IMMUNOLOGICAL METHODS, 2006, 312 (1-2) :167-181
[7]   Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers [J].
Choi, SH ;
Lee, JW ;
Sim, SJ .
BIOSENSORS & BIOELECTRONICS, 2005, 21 (02) :378-383
[8]   Biosensor profiling of molecular interactions in pharmacology [J].
Cooper, MA .
CURRENT OPINION IN PHARMACOLOGY, 2003, 3 (05) :557-562
[9]   Acute pulmonary inflammation induced by exposure of the airways to staphylococcal enterotoxin type B in rats [J].
Desouza, Ivani A. ;
Franco-Penteado, Carla F. ;
Camargo, Enilton A. ;
Lima, Carmen S. P. ;
Teixeira, Simone A. ;
Muscara, Marcelo N. ;
De Nucci, Gilberto ;
Antunes, Edson .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2006, 217 (01) :107-113
[10]   ESTIMATION OF HUMAN DOSE OF STAPHYLOCOCCAL ENTEROTOXIN-A FROM A LARGE OUTBREAK OF STAPHYLOCOCCAL FOOD POISONING INVOLVING CHOCOLATE MILK [J].
EVENSON, ML ;
HINDS, MW ;
BERNSTEIN, RS ;
BERGDOLL, MS .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1988, 7 (04) :311-316