SPR-based immunosensor for determining staphylococcal enterotoxin A

被引：61

作者：

Tsai, Wen-Chi ^{[1
]}

Li, Ie-Chin ^{[1
]}

机构：

[1] Chinese Culture Univ, Grad Inst Biotechnol, Taipei, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 136卷 / 01期

关键词：

Surface plasmon resonance; Mixed self-assembled monolayers; Staphylococcal enterotoxin A; Immunosensor; SURFACE-PLASMON RESONANCE; SELF-ASSEMBLED MONOLAYER; PIEZOELECTRIC CRYSTAL IMMUNOSENSOR; LINKED-IMMUNOSORBENT-ASSAY; ENHANCED PERFORMANCE; BIOSENSOR;

D O I：

10.1016/j.snb.2008.10.061

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immunosensor for determining staphylococcal enterotoxin A (SEA) was developed on the basis of surface plasmon resonance (SPR). The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 16-mercapto-1-hexanol (16-MHA) to 6-mercapto-1-hexanol (6-MCH). The surface functionalized with a 1:20 mixed self-assembled monolayer(SAM) exhibited the best result in terms of SEA detection sensitivity. Through optimization of anti-SEA immobilization, the SPR sensor could detect SEA in phosphate-buffered saline buffer in a linear range from 100 to 1000ng/ml. Furthermore, studies of recovery and matrix effects were performed to evaluate the suitability of the developed immunosensor for directly analyzing SEA in milk samples. Results indicated that the mixed SAM-based SPR immunosensor has the potential for the specific detection of SEA. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：8 / 12

页数：5

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