Plant-derived hemagglutinin protects ferrets against challenge infection with the A/Indonesia/05/05 strain of avian influenza

被引：90

作者：

Shoji, Yoko ^{[1
]}

Bi, Hong ^{[1
]}

Musiychuk, Konstantin ^{[1
]}

Rhee, Amy ^{[1
]}

Horsey, April ^{[1
]}

Roy, Gourgopal ^{[1
]}

Green, Brian ^{[1
]}

Shamloul, Moneim ^{[1
]}

Farrance, Christine E. ^{[1
]}

Taggart, Barbara ^{[2
]}

Mytle, Nutan ^{[2
]}

Ugulava, Natalia ^{[1
]}

Rabindran, Shailaja ^{[1
]}

Mett, Vadim ^{[1
]}

Chichester, Jessica A. ^{[1
]}

Yusibov, Vidadi ^{[1
]}

机构：

[1] Fraunhofer USA Ctr Mol Biotechnol, Newark, DE 19711 USA

[2] So Res Inst, Birmingham, AL 35355 USA

来源：

VACCINE | 2009年 / 27卷 / 07期

关键词：

Highly pathogenic avian influenza; Hemagglutinin; Plant-derived vaccine; RANDOMIZED CONTROLLED-TRIAL; CROSS-REACTIVE IMMUNITY; PANDEMIC INFLUENZA; RECOMBINANT PROTEIN; VACCINE CANDIDATE; VIRAL VECTORS; H5N1; VACCINE; VIRUS; IMMUNOGENICITY; EXPRESSION;

D O I：

10.1016/j.vaccine.2008.11.108

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The global spread of highly pathogenic avian influenza virus (H5N1 subtype) has promoted efforts to develop human vaccines against potential pandemic outbreaks. However, current platforms for influenza vaccine production are cumbersome, limited in scalability and often require the handling of live infectious virus. We describe the production of hemagglutinin from the A/Indonesia/05/05 strain of H5N1 influenza virus by transient expression in plants, and demonstrate the immunogenicity and protective efficacy of the vaccine candidate in animal models. Immunization of mice and ferrets with plant-derived hemagglutinin elicited serum hemagglutinin-inhibiting antibodies and protected the ferrets against challenge infection with a homologous virus. This demonstrates that plant-derived H5 HA is immunogenic in mice and ferrets, and can induce protective immunity against infection with highly pathogenic avian influenza virus. Plants could therefore be Suitable as a platform for the rapid, large-scale production of influenza vaccines in the face of a pandemic. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1087 / 1092

页数：6

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