Use of trichosanthin to reduce infection by turnip mosaic virus

被引:67
作者
Lam, YH
Wong, YS
Wang, B
Wong, RNS
Yeung, HW
Shaw, PC
机构
[1] CHINESE UNIV HONG KONG, DEPT BIOCHEM, HONG KONG, HONG KONG
[2] CHINESE UNIV HONG KONG, DEPT BIOL, HONG KONG, HONG KONG
[3] HONG KONG BAPTIST UNIV, DEPT BIOL, HONG KONG, HONG KONG
关键词
anti-viral assays; Brassica parachinensis; Nicotiana tabacum; ribosome inactivating protein; trichosanthin; turnip mosaic virus;
D O I
10.1016/0168-9452(95)04310-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3' polyadenylation sequence (ocs 3') was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.
引用
收藏
页码:111 / 117
页数:7
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