Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells

被引：249

作者：

Endo, Akira ^{[1
,2
]}

Sawada, Yoshiaki ^{[3
]}

Takahashi, Hirokazu ^{[5
]}

Okamoto, Masanori ^{[1
,2
]}

Ikegami, Keiichi ^{[1
]}

Koiwai, Hanae ^{[1
]}

Seo, Mitsunori ^{[1
]}

Toyomasu, Tomonobu ^{[3
,4
]}

Mitsuhashi, Wataru ^{[3
,4
]}

Shinozaki, Kazuo ^{[6
]}

Nakazono, Mikio ^{[5
]}

Kamiya, Yuji ^{[2
]}

Koshiba, Tomokazu ^{[1
]}

Nambara, Eiji ^{[2
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Biol Sci, Tokyo 1920397, Japan

[2] RIKEN, Plant Sci Ctr, Growth Regulat Res Grp, Kanagawa 2300045, Japan

[3] Iwate Univ, United Grad Sch Agr Sci, Course Sci Bioresource, Morioka, Iwate 0208550, Japan

[4] Yamagata Univ, Dept Bioresource Engn, Yamagata 9978555, Japan

[5] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[6] RIKEN, Plant Sci Ctr, Gene Discovery Res Grp, Kanagawa 2300045, Japan

来源：

PLANT PHYSIOLOGY | 2008年 / 147卷 / 04期

关键词：

D O I：

10.1104/pp.108.116632

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In this study, we examined the tissue-specific localization of ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis ( Arabidopsis thaliana) plants using specific antibodies against 9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3), AtABA2, and Arabidopsis aldehyde oxidase 3 (AAO3). Immunohistochemical analysis revealed that in turgid plants, AtABA2 and AAO3 proteins were localized in vascular parenchyma cells most abundantly at the boundary between xylem and phloem bundles, but the AtNCED3 protein was undetectable in these tissues. In water-stressed plants, AtNCED3 was detected exclusively in the vascular parenchyma cells together with AtABA2 and AAO3. In situ hybridization using the antisense probe for AtNCED3 showed that the drought-induced expression of AtNCED3 was also restricted to the vascular tissues. Expression analysis of laser-microdissected cells revealed that, among nine drought-inducible genes examined, the early induction of most genes was spatially restricted to vascular cells at 1 h and then some spread to mesophyll cells at 3 h. The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight into plant systemic response to drought stresses.

引用

页码：1984 / 1993

页数：10

共 57 条

[21] Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana [J].