Distinctive expression patterns and roles of the miRNA393/TIR1 homolog module in regulating flag leaf inclination and primary and crown root growth in rice (Oryza sativa)

被引:205
作者
Bian, Hongwu [1 ]
Xie, Yakun [1 ]
Guo, Fu [1 ]
Han, Ning [1 ]
Ma, Shengyun [1 ]
Zeng, Zhanghui [1 ]
Wang, Junhui [1 ]
Yang, Yinong [1 ,2 ,3 ]
Zhu, Muyuan [1 ]
机构
[1] Zhejiang Univ, Key Lab Cell & Gene Engn Zhejiang Prov, Inst Genet, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA
[3] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
crown root growth; flag leaf inclination; miRNA393; primary root; rice (Oryza sativa); AUXIN RESPONSE FACTOR; BOX PROTEIN TIR1; COMPLEX REGULATION; AUX/IAA PROTEINS; GENE-EXPRESSION; PLANT TRANSFORMATION; ARABIDOPSIS; MIRNA; IDENTIFICATION; DEGRADATION;
D O I
10.1111/j.1469-8137.2012.04248.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
MicroRNA (miRNA)-mediated regulation of auxin signaling components plays a critical role in plant development. miRNA expression and functional diversity contribute to the complexity of regulatory networks of miRNA/target modules. This study functionally characterizes two members of the rice (Oryza sativa) miR393 family and their target genes, OsTIR1 and OsAFB2 (AUXIN SIGNALING F-BOX), the two closest homologs of Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 (TIR1). <bold></bold> We found that the miR393 family members possess distinctive expression patterns, with miR393a expressed mainly in the crown and lateral root primordia, as well as the coleoptile tip, and miR393b expressed in the shoot apical meristem. Transgenic plants overexpressing miR393a/b displayed a severe phenotype with hallmarks of altered auxin signaling, mainly including enlarged flag leaf inclination and altered primary and crown root growth. Furthermore, OsAFB2- and OsTIR1-suppressed lines exhibited increased inclination of flag leaves at the booting stage, resembling miR393-overexpressing plants. Moreover, yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsTIR1 and OsAFB2 interact with OsIAA1. Expression diversification of miRNA393 implies the potential role of miRNA regulation during species evolution. The conserved mechanisms of the miR393/target module indicate the fundamental importance of the miR393-mediated regulation of auxin signal transduction in rice.
引用
收藏
页码:149 / 161
页数:13
相关论文
共 53 条
[21]   GATEWAY™ vectors for Agrobacterium-mediated plant transformation [J].
Karimi, M ;
Inzé, D ;
Depicker, A .
TRENDS IN PLANT SCIENCE, 2002, 7 (05) :193-195
[22]   The Arabidopsis F-box protein TIR1 is an auxin receptor [J].
Kepinski, S ;
Leyser, O .
NATURE, 2005, 435 (7041) :446-451
[23]   The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a type-A response regulator of cytokinin signaling [J].
Kitomi, Yuka ;
Ito, Hiroko ;
Hobo, Tokunori ;
Aya, Koichiro ;
Kitano, Hidemi ;
Inukai, Yoshiaki .
PLANT JOURNAL, 2011, 67 (03) :472-484
[24]   miRBase: integrating microRNA annotation and deep-sequencing data [J].
Kozomara, Ana ;
Griffiths-Jones, Sam .
NUCLEIC ACIDS RESEARCH, 2011, 39 :D152-D157
[25]   Transcriptome-wide identification of microRNA targets in rice [J].
Li, Yong-Fang ;
Zheng, Yun ;
Addo-Quaye, Charles ;
Zhang, Li ;
Saini, Ajay ;
Jagadeeswaran, Guru ;
Axtell, Michael J. ;
Zhang, Weixiong ;
Sunkar, Ramanjulu .
PLANT JOURNAL, 2010, 62 (05) :742-759
[26]   Complex Regulation of Two Target Genes Encoding SPX-MFS Proteins by Rice miR827 in Response to Phosphate Starvation [J].
Lin, Shu-I ;
Santi, Carole ;
Jobet, Edouard ;
Lacut, Elodie ;
El Kholti, Naima ;
Karlowski, Wojciech M. ;
Verdeil, Jean-Luc ;
Breitler, Jean Christophe ;
Perin, Christophe ;
Ko, Swee-Suak ;
Guiderdoni, Emmanuel ;
Chiou, Tzyy-Jen ;
Echeverria, Manuel .
PLANT AND CELL PHYSIOLOGY, 2010, 51 (12) :2119-2131
[27]   Auxin and Monocot Development [J].
McSteen, Paula .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2010, 2 (03) :a001479
[28]   Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation [J].
Nakagawa, Tsuyoshi ;
Kurose, Takayuki ;
Hino, Takeshi ;
Tanaka, Katsunori ;
Kawamukai, Makoto ;
Niwa, Yasuo ;
Toyooka, Kiminori ;
Matsuoka, Ken ;
Jinbo, Tetsuro ;
Kimura, Tetsuya .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2007, 104 (01) :34-41
[29]   Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in Rice [J].
Nakamura, Ayako ;
Fujioka, Shozo ;
Takatsuto, Suguru ;
Tsujimoto, Masafumi ;
Kitano, Hidemi ;
Yoshida, Shigeo ;
Asami, Tadao ;
Nakano, Takeshi .
PLANT AND CELL PHYSIOLOGY, 2009, 50 (09) :1627-1635
[30]   A plant miRNA contributes to antibacterial resistance by repressing auxin signaling [J].
Navarro, L ;
Dunoyer, P ;
Jay, F ;
Arnold, B ;
Dharmasiri, N ;
Estelle, M ;
Voinnet, O ;
Jones, JDG .
SCIENCE, 2006, 312 (5772) :436-439