An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases

被引：121

作者：

Ceriotti, L ^{[1
]}

de Rooij, NF ^{[1
]}

Verpoorte, E ^{[1
]}

机构：

[1] Univ Neuchatel, Inst Microtechnol, Sensors Actuators & Microsyst Lab, CH-2007 Neuchatel, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 03期

关键词：

D O I：

10.1021/ac0109467

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-mum, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.

引用

页码：639 / 647

页数：9

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