Microbial cell-surface display

被引：418

作者：

Lee, SY

Choi, JH

Xu, ZH

机构：

[1] Korea Adv Inst Sci & Technol, Metab & Biomol Engn Natl Res Lab, Dept Chem & Biomol Engn, Yuseong Gu, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Bioproc Engn Res Ctr, Yuseong Gu, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Ctr Ultramicrochem Proc Syst, Yuseong Gu, Taejon 305701, South Korea

来源：

TRENDS IN BIOTECHNOLOGY | 2003年 / 21卷 / 01期

关键词：

D O I：

10.1016/S0167-7799(02)00006-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring, motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.

引用

页码：45 / 52

页数：8

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