Characterization of silica-based monoliths with bimodal pore size distribution

被引:151
作者
Leinweber, FC
Lubda, D
Cabrera, K
Tallarek, U
机构
[1] Univ Magdeburg, Lehrstuhl Chem Verfahrenstech, D-39106 Magdeburg, Germany
[2] Merck KGAA, R&D Biochem & Separat, D-64293 Darmstadt, Germany
关键词
D O I
10.1021/ac011163o
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Band dispersion was studied and the retention thermodynamics addressed for insulin and angiotensin II on C18 silica monoliths with a bimodal pore size distribution, covering linear mobile-phase velocities up to 1 cm/s and different temperatures. These data suggest that the influence of average column pressure on retention (between 0 and 10 MPa) is not negligible. Plate height curves were interpreted with the van Deemter equation by assuming an independent contribution from mechanical and nonmechanical dispersion mechanisms. This analysis revealed diffusion-limited mass transfer in the mesoporous silica skeleton which, in turn, allowed us to calculate an equivalent dispersion particle diameter (d(disp) = 3 mum) using the C-term parameter of the van Deemter equation. The resulting superposition of reduced plate height curves for monolithic and particulate beds confirmed that this view presents an adequate analogy. The macroporous interskeleton network responsible for the hydraulic permeability of a monolith was translated to the interparticle pore space of particulate beds, and an equivalent permeability particle diameter (d(perm) = 15 mum) was obtained by scaling based on the Kozeny-Carman equation.
引用
收藏
页码:2470 / 2477
页数:8
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