Improvement of photoaffinity SPR Imaging platform and determination of the binding site of p62/SQSTM1 to p38 MAP kinase

被引：17

作者：

Saito, Akiko ^{[1
]}

Kawai, Kayoko ^{[1
,2
]}

Takayama, Hiroshi ^{[1
,2
]}

Sudo, Tatsuhiko ^{[1
]}

Osada, Hiroyuki ^{[1
,2
]}

机构：

[1] RIKEN, Adv Res Inst, Dept Biol Chem, Antibiot Lab, Wako, Saitama 3510198, Japan

[2] Saitama Univ, Grad Sch Sci & Engn, Saitama 3388570, Japan

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2008年 / 3卷 / 8-9期

关键词：

biosensors; gold; peptides; photoaffinity labeling; surface plasmon resonance;

D O I：

10.1002/asia.200800099

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

p38 mitogen-activated protein kinase (MAPK) is a member of the serine/threonine kinases and is activated in response to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock. We revealed in a previous report that p62/SQSTM1, known to participate in proteasomal or autophagosomal protein degradation and cytokine receptor signal transduction pathways, binds to p38 to regulate specifically. Herein, we describe the improvement of the photoaffinity-thiol linker of our SPR imaging platform, which enabled us to determine the binding site of p62 to p38. SPR imaging experiments using a new photoaffinity linker 2 to immobilize the peptides derived from p62 on gold substrate indicate that the domain comprising amino acids 164-190 of p62 binds to p38 directly. These SPR analysis data and empirical biologic data reveal that the binding site of p62 to p38 is the domain corresponding to 173-182.

引用

页码：1607 / 1612

页数：6

共 30 条

[11]

KAWAI K, 2008, J BIOCH

[12] Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique [J].

Kyo, M ;

Yamamoto, T ;

Motohashi, H ;

Kamiya, T ;

Kuroita, T ;

Tanaka, T ;

Engel, JD ;

Kawakami, B ;

Yamamoto, M .

GENES TO CELLS, 2004, 9 (02) :153-164

[13] Quantitative functional analysis of protein complexes on surfaces [J].

Lee, HJ ;

Yan, YL ;

Marriott, G ;

Corn, RM .

JOURNAL OF PHYSIOLOGY-LONDON, 2005, 563 (01) :61-71

[14] Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-κB ligand (RANKL) [J].

Matsumoto, M ;

Sudo, T ;

Saito, T ;

Osada, A ;

Tsujimoto, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (40) :31155-31161

[15] Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition [J].

McDonnell, JM .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (05) :572-577

[16] OXAZOLINES .11. SYNTHESIS OF FUNCTIONALIZED AROMATIC AND ALIPHATIC-ACIDS - USEFUL PROTECTING GROUP FOR CARBOXYLIC-ACIDS AGAINST GRIGNARD AND HYDRIDE REAGENTS [J].

MEYERS, AI ;

TEMPLE, DL ;

HAIDUKEW.D ;

MIHELICH, ED .

JOURNAL OF ORGANIC CHEMISTRY, 1974, 39 (18) :2787-2793

[17] Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers [J].

Miller, JC ;

Zhou, HP ;

Kwekel, J ;

Cavallo, R ;

Burke, J ;

Butler, EB ;

Teh, BS ;

Haab, BB .

PROTEOMICS, 2003, 3 (01) :56-63

[18] Cell signaling and function organized by PB1 domain interactions [J].

Moscat, Jorge ;

Diaz-Meco, Maria T. ;

Albert, Armando ;

Campuzano, Sonsoles .

MOLECULAR CELL, 2006, 23 (05) :631-640

[19] FORMATION OF SELF-ASSEMBLED MONOLAYERS BY CHEMISORPTION OF DERIVATIVES OF OLIGO(ETHYLENE GLYCOL) OF STRUCTURE HS(CH2)11(OCH2CH2)META-OH ON GOLD [J].

PALEGROSDEMANGE, C ;

SIMON, ES ;

PRIME, KL ;

WHITESIDES, GM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (01) :12-20

[20] Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56(lck) and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region [J].

Park, I ;

Chung, J ;

Walsh, CT ;

Yun, YD ;

Strominger, JL ;

Shin, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (26) :12338-12342

← 1 2 3 →