Isolation of all-trans lycopene by high-speed counter-current chromatography using a temperature-controlled solvent system

被引：23

作者：

Baldermann, Susanne ^{[1
]}

Ropeter, Katharina ^{[1
]}

Koehler, Nils ^{[1
]}

Fleischmann, Peter ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, D-38106 Braunschweig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1192卷 / 01期

关键词：

HSCCC; carotenoids; temperature;

D O I：

10.1016/j.chroma.2008.03.084

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of solvent system, partition coefficient, retention of stationary phase, column, revolution speed, and flow rate of mobile phase are well known parameters to effect HSCCC (high-speed counter-current chromatography) separations. Temperature effects on chromatographic techniques like HPLC and GC are well studied, but the influence of temperature on CCC solvent systems is hardly investigated. This paper presents the influence of temperature on several key parameters (partition coefficient, settling time, volume ratios) in the hydrophobic HSCCC solvent system hexane:dichloromethane:acetonitrile (30:11:18, v/v/v) used for the isolation of lycopene from tomato paste at 10, 15, 20 and 25 degrees C. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：191 / 193

页数：3

共 10 条

[1] Isolation of carotenoids from plant materials and dietary supplements by high-speed counter-current chromatography [J].

Aman, R ;

Carle, R ;

Conrad, J ;

Beifuss, U ;

Schieber, A .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1074 (1-2) :99-105

[2]

Berthod A., 2002, Countercurrent Chromatography: The Support-Free Liquid Stationary Phase

[3]

Britton George., 2004, Carotenoids

[4] Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography [J].

Chen, F ;

Li, HB ;

Wong, RNS ;

Ji, B ;

Jiang, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1064 (02) :183-186

[5] Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography [J].

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1065 (02) :145-168

[6] Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health [J].

Khachik, F ;

Carvalho, L ;

Bernstein, PS ;

Muir, GJ ;

Zhao, DY ;

Katz, NB .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2002, 227 (10) :845-851

[7] Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography [J].

Li, HB ;

Chen, F ;

Zhang, TY ;

Yang, FQ ;

Xu, GQ .

JOURNAL OF CHROMATOGRAPHY A, 2001, 905 (1-2) :151-155

[8] Large-scale isolation and purification of scoparone from Herba artemisiae scopariae by high-speed counter-current chromatography [J].

Ma, C. H. ;

Ke, W. ;

Sun, Z. L. ;

Peng, J. Y. ;

Li, Z. X. ;

Zhou, X. ;

Fan, G. R. ;

Huang, C. G. .

CHROMATOGRAPHIA, 2006, 64 (1-2) :83-87

[9] Application of CCC for the separation of lutein from a crude extract of marigold flower petals [J].

Wei, Y ;

Zhang, TY ;

Xu, GQ ;

Ito, Y .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2003, 26 (9-10) :1659-1669

[10] Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste [J].

Wei, Y ;

Zhang, TY ;

Xu, GQ ;

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 2001, 929 (1-2) :169-173

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