Chip-based microsystems for genomic and proteomic analysis

被引：334

作者：

Sanders, GHW ^{[1
]}

Manz, A ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, Zeneca SmithKline Beecham Ctr Analyt Sci, London SW7 2AZ, England

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2000年 / 19卷 / 06期

基金：

英国工程与自然科学研究理事会;

关键词：

micro total analysis systems; DNA; protein; microarrays; microstructures; microfluidics; hybridisation; PCR; sequencing; sizing separations; electrophoresis; immunoassays; mass spectrometry; two-dimensional polyacrylamide electrophoresis;

D O I：

10.1016/S0165-9936(00)00011-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The field of genomics is being immeasurably altered by the introduction of microsystems in the guise of gene microarrays, There are a number of other microfabricated entities in the literature ranging from single chips for PCR to complete analysis systems. Developments in miniaturised protein analysis, though not as yet as revolutionary as some of those for DNA, are progressing. This review aims to describe the present state-of-the-art of microsystems for use in both these fields, this will include bath microarray- (DNA or protein immobilised on chip) and microfluidic- (DNA or proteins being transported, processed and separated in a transport medium) based microdevices, (C)2000 Elsevier Science B.V. All rights reserved.

引用

页码：364 / 378

页数：15

共 96 条

[51]

Larive CK, 1999, ANAL CHEM, V71, p389R, DOI 10.1021/a1990013o

[52] Subattomole-sensitivity microchip nanoelectrospray source with time-of-flight mass spectrometry detection [J].