Chemiluminescent acridinium-9-carboxamide boronic acid probes: Application to a homogenous glycated hemoglobin assay

被引：24

作者：

Adamczyk, M ^{[1
]}

Chen, YY ^{[1
]}

Johnson, DD ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Pan, Y ^{[1
]}

Reddy, RE ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 05期

关键词：

chemiluminescence; homogeneous assay; multivalent ligand; glycated hemoglobin; diabetes marker; boronic acid;

D O I：

10.1016/j.bmcl.2005.11.062

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Chemiluminescent acridinium-9-carboxamide probes containing 1, 3, 9, and 27 phenylboronic acids were prepared and their chemiluminescent properties evaluated. The relative chemiluminescent signal from the probes varied from 4 to 0.83 x 10(19) counts/mol across the series, while the apparent affinity of the probes for the diabetes marker glycated hemoglobin increased from 211 to 0.43 mu M. The dose-dependent modulation of the chemiluminescent intensity of the probes upon binding was used to demonstrate a homogeneous assay for glycated hemoglobin. (C) 2006 Published by Elsevier Ltd.

引用

页码：1324 / 1328

页数：5

共 16 条

[1] Intrinsic factor-mediated modulation of cyanocobalamin-N-sulfonyl-acridinium-9-carboxamide chemiluminescence [J].