Intrinsic factor-mediated modulation of cyanocobalamin-N-sulfonyl-acridinium-9-carboxamide chemiluminescence

被引：3

作者：

Adamczyk, M ^{[1
]}

Johnson, DD ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Pan, Y ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 15期

关键词：

homogeneous chemiluminescent assays; intrinsic factor; vitamin B12; cobalamin;

D O I：

10.1016/j.bmcl.2004.05.062

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Two cyanocobalamin-N-sulfonyl-acridinium-9-carboxamides with linkage through the N-10 or 9-position were prepared from B-12-e-carboxylic acid. The noncovalent association of intrinsic factor with these ligands resulted in specific modulation of the associated chemiluminescence signal either by quenching or changing the emission profile. Either effect was sufficient to formulate a homogeneous assay to detect vitamin B-12 in buffer. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3917 / 3921

页数：5

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