The inconstant gut microbiota of Drosophila species revealed by 16S rRNA gene analysis

被引:239
作者
Wong, Adam C-N [1 ]
Chaston, John M. [1 ]
Douglas, Angela E. [1 ]
机构
[1] Cornell Univ, Dept Entomol, Comstock Hall, Ithaca, NY 14853 USA
关键词
Acetobacter; core microbiota; Drosophila; gut microbiota; Lactobactillus; symbiosis; LACTOBACILLUS-REUTERI; DIVERSITY; COMMUNITY; BACTERIA; EVOLUTION; HOMEOSTASIS; PHYLOGENY; SYMBIOSIS; MODEL; DIET;
D O I
10.1038/ismej.2013.86
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The gut microorganisms in some animals are reported to include a core microbiota of consistently associated bacteria that is ecologically distinctive and may have coevolved with the host. The core microbiota is promoted by positive interactions among bacteria, favoring shared persistence; its retention over evolutionary timescales is evident as congruence between host phylogeny and bacterial community composition. This study applied multiple analyses to investigate variation in the composition of gut microbiota in drosophilid flies. First, the prevalence of five previously described gut bacteria (Acetobacter and Lactobacillus species) in individual flies of 21 strains (10 Drosophila species) were determined. Most bacteria were not present in all individuals of most strains, and bacterial species pairs co-occurred in individual flies less frequently than predicted by chance, contrary to expectations of a core microbiota. A complementary pyrosequencing analysis of 16S rRNA gene amplicons from the gut microbiota of 11 Drosophila species identified 209 bacterial operational taxonomic units (OTUs), with near-saturating sampling of sequences, but none of the OTUs was common to all host species. Furthermore, in both of two independent sets of Drosophila species, the gut bacterial community composition was not congruent with host phylogeny. The final analysis identified no common OTUs across three wild and four laboratory samples of D. melanogaster. Our results yielded no consistent evidence for a core microbiota in Drosophila. We conclude that the taxonomic composition of gut microbiota varies widely within and among Drosophila populations and species. This is reminiscent of the patterns of bacterial composition in guts of some other animals, including humans.
引用
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页码:1922 / 1932
页数:11
相关论文
共 72 条
[1]  
[Anonymous], R LANG ENV STAT COMP
[2]   Midgut Microbiota of the Malaria Mosquito Vector Anopheles gambiae and Interactions with Plasmodium falciparum Infection [J].
Boissiere, Anne ;
Tchioffo, Majoline T. ;
Bachar, Dipankar ;
Abate, Luc ;
Marie, Alexandra ;
Nsango, Sandrine E. ;
Shahbazkia, Hamid R. ;
Awono-Ambene, Parfait H. ;
Levashina, Elena A. ;
Christen, Richard ;
Morlais, Isabelle .
PLOS PATHOGENS, 2012, 8 (05)
[3]   THE ROLES OF HOST EVOLUTIONARY RELATIONSHIPS (GENUS: NASONIA) AND DEVELOPMENT IN STRUCTURING MICROBIAL COMMUNITIES [J].
Brucker, Robert M. ;
Bordenstein, Seth R. .
EVOLUTION, 2012, 66 (02) :349-362
[4]   Drosophila lifespan enhancement by exogenous bacteria [J].
Brummel, T ;
Ching, A ;
Seroude, L ;
Simon, AF ;
Benzer, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (35) :12974-12979
[5]   Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila [J].
Buchon, Nicolas ;
Broderick, Nichole A. ;
Chakrabarti, Sveta ;
Lemaitre, Bruno .
GENES & DEVELOPMENT, 2009, 23 (19) :2333-2344
[6]   Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis [J].
Burke, Catherine ;
Thomas, Torsten ;
Lewis, Matt ;
Steinberg, Peter ;
Kjelleberg, Staffan .
ISME JOURNAL, 2011, 5 (04) :590-600
[7]   Moving pictures of the human microbiome [J].
Caporaso, J. Gregory ;
Lauber, Christian L. ;
Costello, Elizabeth K. ;
Berg-Lyons, Donna ;
Gonzalez, Antonio ;
Stombaugh, Jesse ;
Knights, Dan ;
Gajer, Pawel ;
Ravel, Jacques ;
Fierer, Noah ;
Gordon, Jeffrey I. ;
Knight, Rob .
GENOME BIOLOGY, 2011, 12 (05)
[8]   QIIME allows analysis of high-throughput community sequencing data [J].
Caporaso, J. Gregory ;
Kuczynski, Justin ;
Stombaugh, Jesse ;
Bittinger, Kyle ;
Bushman, Frederic D. ;
Costello, Elizabeth K. ;
Fierer, Noah ;
Pena, Antonio Gonzalez ;
Goodrich, Julia K. ;
Gordon, Jeffrey I. ;
Huttley, Gavin A. ;
Kelley, Scott T. ;
Knights, Dan ;
Koenig, Jeremy E. ;
Ley, Ruth E. ;
Lozupone, Catherine A. ;
McDonald, Daniel ;
Muegge, Brian D. ;
Pirrung, Meg ;
Reeder, Jens ;
Sevinsky, Joel R. ;
Tumbaugh, Peter J. ;
Walters, William A. ;
Widmann, Jeremy ;
Yatsunenko, Tanya ;
Zaneveld, Jesse ;
Knight, Rob .
NATURE METHODS, 2010, 7 (05) :335-336
[9]   IDENTIFICATION OF APHID (HOMOPTERA, APHIDIDAE) SPECIES AND CLONES BY RANDOM AMPLIFIED POLYMORPHIC DNA [J].
CENIS, JL ;
PEREZ, P ;
FERERES, A .
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1993, 86 (05) :545-550
[10]   Bacterial Communities of Diverse Drosophila Species: Ecological Context of a Host-Microbe Model System [J].
Chandler, James Angus ;
Lang, Jenna Morgan ;
Bhatnagar, Srijak ;
Eisen, Jonathan A. ;
Kopp, Artyom .
PLOS GENETICS, 2011, 7 (09)