Efficient in vivo priming of specific cytotoxic T cell responses by neonatal dendritic cells

被引:47
作者
Dadaglio, G
Sun, CM
Lo-Man, R
Siegrist, CA
Leclerc, C
机构
[1] Inst Pasteur, Unit Biol Immune Regulat, F-75724 Paris 15, France
[2] Univ Geneva, World Hlth Org, Collaborating Ctr Neonatal Vaccinol, Geneva, Switzerland
关键词
D O I
10.4049/jimmunol.168.5.2219
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In early life, a high susceptibility to infectious diseases as well as a poor capacity to respond to vaccines are generally observed as compared with observations in adults. The mechanisms underlying immune immaturity have not been fully elucidated and could be due to the immaturity of the TB cell responses and/or to a defect in the nature and quality of Ag presentation by the APC. This prompted us to phenotypically and functionally characterize early life murine dendritic cells (DC) purified from spleens of 7-day-old mice. We showed that neonatal CD11c(+) DC express levels of costimulatory molecules and MHC molecules similar to those of adult DC and are able to fully maturate after LPS activation. Furthermore, we demonstrated that neonatal DC can efficiently take up, process, and present Ag to T cells in vitro and induce specific CTL responses in vivo. Although a reduced number of these cells was observed in the spleen of neonatal mice as compared with adults, this study clearly shows that neonatal DC have full functional capacity and may well prime Ag-specific naive T cells in vivo.
引用
收藏
页码:2219 / 2224
页数:6
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