Peptide-Functionalized, Low-Biofouling Click Multilayers for Promoting Cell Adhesion and Growth

被引：49

作者：

Kinnane, Cameron R. ^{[1
]}

Wark, Kim ^{[2
]}

Such, Georgina K. ^{[1
]}

Johnston, Angus P. R. ^{[1
]}

Caruso, Frank ^{[1
]}

机构：

[1] Univ Melbourne, Dept Chem & Biomol Engn, Ctr Nanosci & Nanotechnol, Parkville, Vic 3010, Australia

[2] CSIRO, Parkville, Vic 3052, Australia

来源：

SMALL | 2009年 / 5卷 / 04期

关键词：

biocompatible materials; cells; multilayers; peptides; thin films; POLYMERS; CHEMISTRY; ULTRATHIN; FILMS; FIBRONECTIN; ATTACHMENT; RGD;

D O I：

10.1002/smll.200801012

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The preparation of low-fouling poly(ethylene glycol) (PEG) acrylate multilayers through the click-layer-by-layer (LbL) technique was reported. Radical polymerization was used to synthesize PEG-acrylate polymers with alkyne or azide functionalities. Nuclear magnetic resonance (NMR) spectroscopy show that PEG-Az contain approximately 15% azide groups and PEG-Alk 40% alkyne groups. It is also found that peptides can be coupled to the PEGylated surface by the attachment of GRGDSP-propargyl Gly, through analysis by ellipsometry. The potential of the PEG films to provide a low-biofouling surface assessed by comparing cell adhesion on untreated glass and PEG multilayers show that these cell surfaces have an elongated or flattened profile, exhibiting epithelial morphology. A steady growth of cells is observed on the untreated glass surface over 72 h, while a slight increase in the number of cells is seen for the PEG films.

引用

页码：444 / 448

页数：5

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