Zili is required for germ cell differentiation and meiosis in zebrafish

被引：240

作者：

Houwing, Saskia ^{[1
]}

Berezikov, Eugene ^{[1
]}

Ketting, Rene F. ^{[1
]}

机构：

[1] Royal Netherlands Acad Arts & Sci, Univ Med Ctr Utrecht, Hubrecht Inst, NL-3584 CT Utrecht, Netherlands

来源：

EMBO JOURNAL | 2008年 / 27卷 / 20期

关键词：

germ cell differentiation; meiosis; piRNAs; Piwi; zebrafish;

D O I：

10.1038/emboj.2008.204

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small RNAs exert an effect through diverse RNA interference pathways to transcriptionally or post-transcriptionally silence their targets. The Piwi-interacting RNAs (piRNAs) represent a germline-specific small RNA pathway where Piwi proteins themselves are thought to mediate piRNA biosynthesis. Here, we provide strong evidence for a piRNA amplification loop in zebrafish, in which Ziwi and Zili bind piRNAs of opposite polarity. Furthermore, we describe a function for Zili in transposon defense and germ cell differentiation, as well as a crucial function in meiosis, significantly extending the function of Piwi proteins beyond the control of transposable elements in vertebrates.

引用

页码：2702 / 2711

页数：10

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