Signal amplification by substrate recycling on polyaniline/lactate oxidase/lactate dehydrogenase bienzyme electrodes

被引：20

作者：

Chaubey, A

Pande, KK

Pandey, MK

Singh, VS

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] LLRM Med Coll, Dept Biochem, Meerut 250004, Uttar Pradesh, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2001年 / 96卷 / 1-3期

关键词：

lactate oxidase; lactate dehydrogenase; substrate recycling; coimmobilization; bienzyme electrode;

D O I：

10.1385/ABAB:96:1-3:239

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate at lower concentrations (0.1-1 mM). The PANI/LOD/LDH bienzyme electrodes were found to be stable for about 21 d at 4-10 degreesC.

引用

页码：239 / 248

页数：10

共 28 条

[1] ENZYMATIC-SYNTHESIS OF POLYANILINE FILM USING A COPPER-CONTAINING OXIDOREDUCTASE - BILIRUBIN OXIDASE [J].