Increase of catalytic activity of α-chymotrypsin in organic solvent by co-lyophilization with cyclodextrins

被引：19

作者：

Ooe, Y ^{[1
]}

Yamamoto, S ^{[1
]}

Kobayashi, M ^{[1
]}

Kise, H ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Mat Sci, Ibaraki, Osaka 3058573, Japan

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 05期

关键词：

alpha-chymotrypsin; cyclodextrin; co-lyophilization; organic solvent; transesterification;

D O I：

10.1023/A:1005447407774

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-Chymotrypsin was lyophilized in the presence of 2,3,6-tri-O-methyl beta-cyclodextrin, and it displayed activity 40 fold higher than free alpha-chymotrypsin for transesterification in acetonitrile. alpha-Chymotrypsin which was co-lyophilized with hydroxypropylated beta- or gamma-cyclodextrins retained more than 98% of its initial activity after 6 h incubation in acetonitrile.

引用

页码：385 / 389

页数：5

共 8 条

[1] Biologically active peptides and enzymatic approaches to their production [J].

Gill, I ;

LopezFandino, R ;

Jorba, X ;

Vulfson, EN .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (03) :162-183

[2] THERMODYNAMIC PREDICTIONS FOR BIOCATALYSIS IN NONCONVENTIONAL MEDIA - THEORY, TESTS, AND RECOMMENDATIONS FOR EXPERIMENTAL-DESIGN AND ANALYSIS [J].

HALLING, PJ .

ENZYME AND MICROBIAL TECHNOLOGY, 1994, 16 (03) :178-206

[3] ENZYMATIC PEPTIDE-SYNTHESIS VIA SEGMENT CONDENSATION IN THE PRESENCE OF WATER MIMICS [J].

KITAGUCHI, H ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (26) :9272-9273

[4] USE OF CYCLODEXTRINS IN ENZYMOLOGY TO ENHANCE THE SOLUBILITY OF HYDROPHOBIC COMPOUNDS IN WATER [J].

OTERO, C ;

CRUZADO, C ;

BALLESTEROS, A .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1991, 27 (02) :185-194

[5] Subtilisin catalysis of substrate anchored in cyclodextrin [J].

Prabhu, KS ;

Ramadoss, CS .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 69 (01) :69-77

[6] ORGANIC-SOLVENTS FOR BIOORGANIC SYNTHESIS .1. OPTIMIZATION OF PARAMETERS FOR A CHYMOTRYPSIN CATALYZED PROCESS [J].

RESLOW, M ;

ADLERCREUTZ, P ;

MATTIASSON, B .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1987, 26 (01) :1-8

[7] EFFECTS OF CALCIUM-ION ON THE CATALYTIC ACTIVITY OF ALPHA-CHYMOTRYPSIN IN ORGANIC-SOLVENTS [J].

SASAKI, T ;

KISE, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (06) :1050-1053

[8] CATALYSIS OF ENZYME AGGREGATES IN ORGANIC-SOLVENTS - AN ATTEMPT AT EVALUATION OF INTRINSIC ACTIVITY OF PROTEASES IN ETHANOL [J].

YAMAMOTO, Y ;

KISE, H .

BIOTECHNOLOGY LETTERS, 1993, 15 (06) :647-652

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