Nanostructured zinc oxide film for urea sensor

被引:104
作者
Ali, Azahar [1 ]
Ansari, Anees A. [1 ]
Kaushik, Ajeet [1 ]
Solanki, Pratima R. [1 ]
Barik, A. [1 ]
Pandey, M. K. [1 ]
Malhotra, B. D. [1 ]
机构
[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India
关键词
Nanostructured ZnO; Nanomaterials; Sensor; Electrochemical deposition; Enzyme; HORSERADISH-PEROXIDASE; ZNO; IMMOBILIZATION; MATRIX;
D O I
10.1016/j.matlet.2009.08.038
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Nanostructured zinc oxide (Nano-ZnO) film has been electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate to co-immobilized urease (Urs) and glutamate dehydrogenase (GLDH) for urea detection. The observed reflection planes corresponding to wurtzite ZnO nanoparticles (similar to 25 nm) in XRD diffraction pattern and UV-visible absorption band at 338 nm reveal the formation of Nano-ZnO. Urs-GLDH/Nano-ZnO/ITO bioelectrode shows high sensitivity for urea detection within 10-80 mg/dL and limit of detection as 13.5 mg/dL with regression coefficient as 0.994 and Michaelis-Menten constant (K-m, 6.1 mg/dL) indicating good affinity of Urs-GLDH to urea. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:2473 / 2475
页数:3
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