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Nanostructured zinc oxide film for urea sensor
被引:104
作者:

Ali, Azahar
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Ansari, Anees A.
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Kaushik, Ajeet
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Solanki, Pratima R.
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Barik, A.
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Pandey, M. K.
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

Malhotra, B. D.
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Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India
机构:
[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India
关键词:
Nanostructured ZnO;
Nanomaterials;
Sensor;
Electrochemical deposition;
Enzyme;
HORSERADISH-PEROXIDASE;
ZNO;
IMMOBILIZATION;
MATRIX;
D O I:
10.1016/j.matlet.2009.08.038
中图分类号:
T [工业技术];
学科分类号:
08 ;
摘要:
Nanostructured zinc oxide (Nano-ZnO) film has been electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate to co-immobilized urease (Urs) and glutamate dehydrogenase (GLDH) for urea detection. The observed reflection planes corresponding to wurtzite ZnO nanoparticles (similar to 25 nm) in XRD diffraction pattern and UV-visible absorption band at 338 nm reveal the formation of Nano-ZnO. Urs-GLDH/Nano-ZnO/ITO bioelectrode shows high sensitivity for urea detection within 10-80 mg/dL and limit of detection as 13.5 mg/dL with regression coefficient as 0.994 and Michaelis-Menten constant (K-m, 6.1 mg/dL) indicating good affinity of Urs-GLDH to urea. (C) 2009 Elsevier B.V. All rights reserved.
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页码:2473 / 2475
页数:3
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