Nanostructured zinc oxide platform for cholesterol sensor

被引：99

作者：

Solanki, Pratima R. ^{[1
]}

Kaushik, Ajeet ^{[1
]}

Ansari, Anees A. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Ctr Biomol Elect, Natl Phys Lab, Dept Sci & Technol, New Delhi 110012, India

来源：

APPLIED PHYSICS LETTERS | 2009年 / 94卷 / 14期

关键词：

biochemistry; biosensors; charge exchange; electrochemical electrodes; electrochemical sensors; enzymes; II-VI semiconductors; molecular biophysics; nanobiotechnology; nanofabrication; reaction rate constants; semiconductor thin films; sol-gel processing; voltammetry (chemical analysis); wide band gap semiconductors; zinc compounds; ZNO NANOPARTICLES; BIOSENSOR; FILM;

D O I：

10.1063/1.3111429

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nanostructured zinc oxide (nano-ZnO) film has been fabricated onto indium tin oxide (ITO) containing preferred (002) plane and 10 nm crystallite size using sol-gel technique for immobilization of cholesterol oxidase (ChOx). Electrochemical response of ChOx/nano-ZnO/ITO bioelectrode determined as a function of cholesterol concentration using cyclic voltammetry technique reveals improved detection range (5-400 mg/dl), low detection limit (0.5 mg/dl), fast response time (10 s), sensitivity (0.059 mu A/mg dl(-1) cm(-2)), and low value (0.98 mg/dl) of Michaelis-Menten constant (K-m). It is shown that nano-ZnO film provides better environment and enhanced electron transfer between ChOx and electrode.

引用

页数：3

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