A novel transposon for functional expression of DNA libraries

被引：10

作者：

Leggewie, Christian

Henning, Helge

Schmeisser, Christel

Streit, Wolfgang R.

Jaeger, Karl-Erich ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Mol Enzymtechnol, Forschungszentrum Juelich, D-52426 Julich, Germany

[2] Univ Duisburg Essen, Biofilm Ctr, AG Mol Enzymtechnol, D-47057 Duisburg, Germany

来源：

JOURNAL OF BIOTECHNOLOGY | 2006年 / 123卷 / 03期

关键词：

transposon MuExpress; metagenome; biocatalysts; activity screening;

D O I：

10.1016/j.jbiotec.2005.12.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Environmental DNA libraries are important sources for novel biocatalyst genes but activity screening for relevant enzymes is often inefficient. Therefore, we have constructed the transposon MuExpress which randomly integrates in vitro into existing bacterial artificial chromosome (BAC) or cosmid libraries and permits the inducible expression of its flanking regions in both directions. Furthermore, this transposon allows the bidirectional sequencing of the respective clones starting from unique primer binding sites. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：281 / 287

页数：7

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