Random distribution of biomaterials as a handling method on microarray applied to PCR, biosensors and high through-put screening

被引:2
作者
Murakami, Y [1 ]
Nagai, H [1 ]
Kikuchi, T [1 ]
Yamamura, A [1 ]
Idegami, K [1 ]
Yanase, M [1 ]
Choi, YS [1 ]
Morita, Y [1 ]
Tamiya, E [1 ]
机构
[1] Japan Adv Inst Sci & Technol, Tatsunokuchi, Ishikawa 9231292, Japan
来源
1ST ANNUAL INTERNATIONAL IEEE-EMBS SPECIAL TOPIC CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE & BIOLOGY, PROCEEDINGS | 2000年
关键词
D O I
10.1109/MMB.2000.893734
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
This paper describes a random distribution method to bring biomaterials into an array of sites on a chip. A mixture of biomolecules or a mixed suspension of particles immobilized with biomaterials; was simply poured on an array and the components were randomly distributed. A microchamber array for PCR was developed by semiconductor microfabrication techniques. Amplification of pGFP was confirmed using a technique based on energy transfer of fluorescent dyes. Addition of BSA in PCR mixture enhances PCR efficiency. Furthermore, employing a special membrane made it possible to pick up the PCR products. Random distribution method is also applied to the construction of biosensor arrays. We first employed a two-step immobilization in which materials are not immobilized directly but indirectly via small support materials. In this case random distribution is called random fluidic self-assembly. This arranges the microparticles reducing the complication in the second step on the chip.
引用
收藏
页码:29 / 32
页数:4
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