Behind the scenes of a small RNA gene-silencing pathway

被引：17

作者：

Ku, Gregory ^{[1
,2
]}

McManus, Michael T. ^{[1
,3
]}

机构：

[1] Univ Calif San Francisco, UCSF Diabet Ctr, San Francisco, CA 94122 USA

[2] Univ Calif San Francisco, Dept Med, Div Endocrinol & Metab, San Francisco, CA 94122 USA

[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94122 USA

来源：

HUMAN GENE THERAPY | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1089/hum.2007.1226

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference (RNAi) is a sequence-specific gene-silencing phenomenon mediated by double-stranded RNA. RNAi is made possible through the activity of an evolutionarily conserved RNA-protein machinery that is utilized by microRNAs (miRNAs), endogenously produced 21- to 23-nucleotide noncoding RNAs. As RNA-based therapeutics come of age, understanding the miRNA pathway and the mechanism of posttranscriptional gene silencing is paramount. Studies have pointed to a critical role for miRNAs in human development and disease and revealed an unanticipated complexity in the mechanisms of regulation of gene expression. This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA- based treatments.

引用

页码：17 / 26

页数：10

共 119 条

[1] Drosophila microRNAs exhibit diverse spatial expression patterns during embryonic development
Aboobaker, AA
Tomancak, P
Patel, N
Rubin, GM
Lai, EC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (50) : 18017 - 18022
[2] Molecular basis for target RNA recognition and cleavage by human RISC
Ameres, Stefan Ludwig
Martinez, Javier
Schroeder, Renee
[J]. CELL, 2007, 130 (01) : 101 - 112
[3] Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation
Bagga, S
Bracht, J
Hunter, S
Massirer, K
Holtz, J
Eachus, R
Pasquinelli, AE
[J]. CELL, 2005, 122 (04) : 553 - 563
[4] Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes
Baskerville, S
Bartel, DP
[J]. RNA, 2005, 11 (03) : 241 - 247
[5] MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes
Behm-Ansmant, Isabelle
Rehwinkel, Jan
Doerks, Tobias
Stark, Alexander
Bork, Peer
Izaurralde, Elisa
[J]. GENES & DEVELOPMENT, 2006, 20 (14) : 1885 - 1898
[6] Identification of hundreds of conserved and nonconserved human microRNAs
Bentwich, I
Avniel, A
Karov, Y
Aharonov, R
Gilad, S
Barad, O
Barzilai, A
Einat, P
Einav, U
Meiri, E
Sharon, E
Spector, Y
Bentwich, Z
[J]. NATURE GENETICS, 2005, 37 (07) : 766 - 770
[7] Mammalian mirtron genes
Berezikov, Eugene
Chung, Wei-Jen
Willis, Jason
Cuppen, Edwin
Lai, Eric C.
[J]. MOLECULAR CELL, 2007, 28 (02) : 328 - 336
[8] Diversity of microRNAs in human and chimpanzee brain
Berezikov, Eugene
Thuemmler, Fritz
van Laake, Linda W.
Kondova, Ivanela
Bontrop, Ronald
Cuppen, Edwin
Plasterk, Ronald H. A.
[J]. NATURE GENETICS, 2006, 38 (12) : 1375 - 1377
[9] Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
Berezikov, Eugene
van Tetering, Geert
Verheul, Mark
van de Belt, Jose
van Laake, Linda
Vos, Joost
Verloop, Robert
van de Wetering, Marc
Guryev, Victor
Takada, Shuji
van Zonneveld, Anton Jan
Mano, Hiroyuki
Plasterk, Ronald
Cuppen, Edwin
[J]. GENOME RESEARCH, 2006, 16 (10) : 1289 - 1298
[10] Dicer is essential for mouse development
Bernstein, E
Kim, SY
Carmell, MA
Murchison, EP
Alcorn, H
Li, MZ
Mills, AA
Elledge, SJ
Anderson, KV
Hannon, GJ
[J]. NATURE GENETICS, 2003, 35 (03) : 215 - 217

← 1 2 3 4 5 6 7 8 9 10 →