An Airborne Transmissible Avian Influenza H5 Hemagglutinin Seen at the Atomic Level

被引：121

作者：

Zhang, Wei ^{[1
,3
]}

Shi, Yi ^{[1
,2
]}

Lu, Xishan ^{[4
]}

Shu, Yuelong ^{[5
]}

Qi, Jianxun ^{[1
]}

Gao, George F. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth RNIH, Beijing 100101, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

[5] Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

SCIENCE | 2013年 / 340卷 / 6139期

基金：

中国国家自然科学基金;

关键词：

A VIRUSES; RECEPTOR-BINDING; SWINE-ORIGIN;

D O I：

10.1126/science.1236787

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent studies have identified several mutations in the hemagglutinin (HA) protein that allow the highly pathogenic avian H5N1 influenza A virus to transmit between mammals by airborne route. Here, we determined the complex structures of wild-type and mutant HAs derived from an Indonesia H5N1 virus bound to either avian or human receptor sialic acid analogs. A cis/trans conformational change in the glycosidic linkage of the receptor analog was observed, which explains how the H5N1 virus alters its receptor-binding preference. Furthermore, the mutant HA possessed low affinities for both avian and human receptors. Our findings provide a structural and biophysical basis for the H5N1 adaptation to acquire human, but maintain avian, receptor-binding properties.

引用

页码：1463 / 1467

页数：5

共 25 条

[1] Current concepts - Avian influenza A (H5N1) infection in humans [J].

Beigel, H ;

Farrar, H ;

Han, AM ;

Hayden, FG ;

Hyer, R ;

de Jong, MD ;

Lochindarat, S ;

Tien, NTK ;

Hien, NT ;

Hien, TT ;

Nicoll, A ;

Touch, S ;

Yuen, KY .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (13) :1374-1385

[2] First introduction of highly pathogenic H5NI avian influenza A viruses in wild and domestic birds in Denmark, Northern Europe [J].

Bragstad, Karoline ;

Jorgensen, Poul H. ;

Handberg, Kurt ;

Hammer, Anne S. ;

Kabell, Susanne ;

Fomsgaard, Anders .

VIROLOGY JOURNAL, 2007, 4 (1)

[3] Summary of Avian Influenza Activity in Europe, Asia, and Africa, 2006-2009 [J].

Brown, Ian H. .

AVIAN DISEASES, 2010, 54 (01) :187-193

[4] In Vitro Assessment of Attachment Pattern and Replication Efficiency of H5N1 Influenza A Viruses with Altered Receptor Specificity [J].

Chutinimitkul, Salin ;

van Riel, Debby ;

Munster, Vincent J. ;

van den Brand, Judith M. A. ;

Rimmelzwaan, Guus F. ;

Kuiken, Thijs ;

Osterhaus, Albert D. M. E. ;

Fouchier, Ron A. M. ;

de Wit, Emmie .

JOURNAL OF VIROLOGY, 2010, 84 (13) :6825-6833

[5] Binding of the influenza A virus to cell-surface receptors: Structures of five hemagglutinin-sialyloligosaccharide complexes determined by x-ray crystallography [J].

Eisen, MB ;

Sabesan, S ;

Skehel, JJ ;

Wiley, DC .

VIROLOGY, 1997, 232 (01) :19-31

[6] The structure and receptor binding properties of the 1918 influenza hemagglutinin [J].

Gamblin, SJ ;

Haire, LF ;

Russell, RJ ;

Stevens, DJ ;

Xiao, B ;

Ha, Y ;

Vasisht, N ;

Steinhauer, DA ;

Daniels, RS ;

Elliot, A ;

Wiley, DC ;

Skehel, JJ .

SCIENCE, 2004, 303 (5665) :1838-1842

[7] Influenza Hemagglutinin and Neuraminidase Membrane Glycoproteins [J].

Gamblin, Steven J. ;

Skehel, John J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (37) :28403-28409

[8]

Gao R., 2013, NEW ENGL J MED, V368, P1888

[9] Identification of Amino Acids in HA and PB2 Critical for the Transmission of H5N1 Avian Influenza Viruses in a Mammalian Host [J].

Gao, Yuwei ;

Zhang, Ying ;

Shinya, Kyoko ;

Deng, Guohua ;

Jiang, Yongping ;

Li, Zejun ;

Guan, Yuntao ;

Tian, Guobin ;

Li, Yanbing ;

Shi, Jianzhong ;

Liu, Liling ;

Zeng, Xianying ;

Bu, Zhigao ;

Xia, Xianzhu ;

Kawaoka, Yoshihiro ;

Chen, Hualan .

PLOS PATHOGENS, 2009, 5 (12)

[10] Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans [J].

Garten, Rebecca J. ;

Davis, C. Todd ;

Russell, Colin A. ;

Shu, Bo ;

Lindstrom, Stephen ;

Balish, Amanda ;

Sessions, Wendy M. ;

Xu, Xiyan ;

Skepner, Eugene ;

Deyde, Varough ;

Okomo-Adhiambo, Margaret ;

Gubareva, Larisa ;

Barnes, John ;

Smith, Catherine B. ;

Emery, Shannon L. ;

Hillman, Michael J. ;

Rivailler, Pierre ;

Smagala, James ;

de Graaf, Miranda ;

Burke, David F. ;

Fouchier, Ron A. M. ;

Pappas, Claudia ;

Alpuche-Aranda, Celia M. ;

Lopez-Gatell, Hugo ;

Olivera, Hiram ;

Lopez, Irma ;

Myers, Christopher A. ;

Faix, Dennis ;

Blair, Patrick J. ;

Yu, Cindy ;

Keene, Kimberly M. ;

Dotson, P. David, Jr. ;

Boxrud, David ;

Sambol, Anthony R. ;

Abid, Syed H. ;

George, Kirsten St. ;

Bannerman, Tammy ;

Moore, Amanda L. ;

Stringer, David J. ;

Blevins, Patricia ;

Demmler-Harrison, Gail J. ;

Ginsberg, Michele ;

Kriner, Paula ;

Waterman, Steve ;

Smole, Sandra ;

Guevara, Hugo F. ;

Belongia, Edward A. ;

Clark, Patricia A. ;

Beatrice, Sara T. ;

Donis, Ruben .

SCIENCE, 2009, 325 (5937) :197-201

← 1 2 3 →