Extracellular Matrix Functionalized Microcavities to Control Hematopoietic Stem and Progenitor Cell Fate

被引：33

作者：

Kurth, Ina ^{[1
]}

Franke, Katja ^{[1
]}

Pompe, Tilo ^{[1
]}

Bornhaeuser, Martin ^{[2
]}

Werner, Carsten ^{[1
,3
]}

机构：

[1] Leibniz Inst Polymer Res Dresden, Max Bergmann Ctr Biomat Dresden, D-01069 Dresden, Germany

[2] Univ Klinikum Carl Gustav Carus, Med Klin & Poliklin 1, D-01307 Dresden, Germany

[3] Ctr Regenerat Therapies Dresden, D-01307 Dresden, Germany

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 06期

关键词：

bioengineering; microstructures; proteins; stem cells; IN-VITRO; HEPARAN SULFATES; ADHESION; BONE; FIBRONECTIN; NICHES; RECEPTORS; PROTEINS; MODEL;

D O I：

10.1002/mabi.201000432

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymeric microcavities functionalized with extracellular matrix components were used as an experimental in vitro model to investigate principles of hematopoietic stem and progenitor cell (HSPC) fate control. Using human CD133+ HSPC we could demonstrate distinct differences in HSPC cycling and differentiation dependence on the adhesion ligand specificity (i.e., heparin, collagen I) and cytokine levels. The presented microcavity platform provides a powerful in vitro approach to explore the role of exogenous cues in HSPC fate decisions and can therefore be instrumental to progress in stem cell biology and translational research toward new therapies.

引用

页码：739 / 747

页数：9

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