Stepwise Histone Replacement by SWR1 Requires Dual Activation with Histone H2A.Z and Canonical Nucleosome

被引:168
作者
Luk, Ed [1 ]
Ranjan, Anand [1 ]
FitzGerald, Peter C. [2 ]
Mizuguchi, Gaku [1 ]
Huang, Yingzi [1 ]
Wei, Debbie [1 ]
Wu, Carl [1 ]
机构
[1] NCI, Biochem & Mol Biol Lab, NIH, Bethesda, MD 20892 USA
[2] NCI, Genome Anal Unit, NIH, Bethesda, MD 20892 USA
关键词
VARIANT H2A.Z; CHROMATIN; TRANSCRIPTION; REPLICATION; PROMOTERS; EXCHANGE; COMPLEX; ACETYLATION; STABILITY; DYNAMICS;
D O I
10.1016/j.cell.2010.10.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Histone variant H2A.Z-containing nucleosomes are incorporated at most eukaryotic promoters. This incorporation is mediated by the conserved SWR1 complex, which replaces histone H2A in canonical nucleosomes with H2A.Z in an ATP-dependent manner. Here, we show that promoter-proximal nucleosomes are highly heterogeneous for H2A.Z in Saccharomyces cerevisiae, with substantial representation of nucleosomes containing one, two, or zero H2A.Z molecules. SWR1-catalyzed H2A.Z replacement in vitro occurs in a stepwise and unidirectional fashion, one H2A.Z-H2B dimer at a time, producing heterotypic nucleosomes as intermediates and homotypic H2A.Z nucleosomes as end products. The ATPase activity of SWR1 is specifically stimulated by H2A-containing nucleosomes without ensuing histone H2A eviction. Remarkably, further addition of free H2A.Z-H2B dimer leads to hyperstimulation of ATPase activity, eviction of nucleosomal H2A-H2B, and deposition of H2A.Z-H2B. These results suggest that the combination of H2A-containing nucleosome and free H2A.Z-H2B dimer acting as both effector and substrate for SWR1 governs the specificity and outcome of the replacement reaction.
引用
收藏
页码:725 / 736
页数:12
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