Quantitative measurement of single cell dynamics

被引：27

作者：

Bakstad, Denise ^{[1
]}

Adamson, Antony ^{[1
]}

Spiller, David G. ^{[1
]}

White, Michael R. H. ^{[1
]}

机构：

[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2012年 / 23卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

FLUORESCENT PROTEINS; GENE-EXPRESSION; RNAI SCREEN; TRANSCRIPTION; QUANTIFICATION; MOUSE;

D O I：

10.1016/j.copbio.2011.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the past 20 years luminescent and fluorescent imaging assays have been developed to report on the dynamics of transcription and protein translocation in single cells. The combination of these measurements with mathematical analysis is having an increasingly significant impact on cell biology. There is an urgent need to translate these assays to the study of cells and tissues in vivo, which requires new tools and technologies. Emergence of these new tools and techniques will further the understanding of the role of signalling and transcriptional dynamics in the generation of cellular heterogeneity and the control of cell fate.

引用

页码：103 / 109

页数：7

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