Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents

被引：42

作者：

De León, A

García, B

de la Rosa, APB

Villaseñor, F

Estrada, A

López-Revilla, R

机构：

[1] Inst Potosino Invest Cientif & Tecnol, Dept Mol Biol, San Luis Potosi 78231, SLP, Mexico

[2] Inst Tecnol Celaya, Celaya 38040, Gto, Mexico

[3] Univ Autonoma San Luis Potosi, San Luis Potosi 78210, SLP, Mexico

来源：

PROCESS BIOCHEMISTRY | 2003年 / 39卷 / 03期

关键词：

organic solvent; penneabilization; penicillin G acylase; dielectric constant; Hydrophobicity; recombinant; E; coli;

D O I：

10.1016/S0032-9592(03)00079-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant Escherichia coli cells expressing penicillin G acylase (PGA) were permeabilized with organic solvents. Using the aromatic solvents, hexane or chloroform, increases of specific enzyme activity up to 380% were obtained, whereas acetone and pyridine reduced drastically activity and alcohols inactivated the enzyme. The type of solvent, physicochemical properties, solvent concentration and time of permeabilization were evaluated to obtain the optimal conditions to increase PGA activity by cell permeabilization. Solvents with lower dielectric constants (<5) and high hydrophobicity (log P > 2) were more effective in increasing PGA activity. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：301 / 305

页数：5

共 25 条

[1] SOLVENT DIELECTRIC EFFECTS ON PROTEIN DYNAMICS [J].