Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents

被引：42

作者：

De León, A

García, B

de la Rosa, APB

Villaseñor, F

Estrada, A

López-Revilla, R

机构：

[1] Inst Potosino Invest Cientif & Tecnol, Dept Mol Biol, San Luis Potosi 78231, SLP, Mexico

[2] Inst Tecnol Celaya, Celaya 38040, Gto, Mexico

[3] Univ Autonoma San Luis Potosi, San Luis Potosi 78210, SLP, Mexico

来源：

PROCESS BIOCHEMISTRY | 2003年 / 39卷 / 03期

关键词：

organic solvent; penneabilization; penicillin G acylase; dielectric constant; Hydrophobicity; recombinant; E; coli;

D O I：

10.1016/S0032-9592(03)00079-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant Escherichia coli cells expressing penicillin G acylase (PGA) were permeabilized with organic solvents. Using the aromatic solvents, hexane or chloroform, increases of specific enzyme activity up to 380% were obtained, whereas acetone and pyridine reduced drastically activity and alcohols inactivated the enzyme. The type of solvent, physicochemical properties, solvent concentration and time of permeabilization were evaluated to obtain the optimal conditions to increase PGA activity by cell permeabilization. Solvents with lower dielectric constants (<5) and high hydrophobicity (log P > 2) were more effective in increasing PGA activity. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：301 / 305

页数：5

相关论文

共 25 条

[11] BIOCATALYSIS IN MULTIPHASE REACTION MIXTURES CONTAINING ORGANIC LIQUIDS [J].

HALLING, PJ .

BIOTECHNOLOGY ADVANCES, 1987, 5 (01) :47-84

[12] Studies on lactate dehydrogenase of Lactobacillus plantarum spp. involved in lactic acid biosynthesis using permeabilized cells [J].

Krishnan, S ;

Gowda, LR ;

Karanth, NG .

PROCESS BIOCHEMISTRY, 2000, 35 (10) :1191-1198

[13] RULES FOR OPTIMIZATION OF BIOCATALYSIS IN ORGANIC-SOLVENTS [J].

LAANE, C ;

BOEREN, S ;

VOS, K ;

VEEGER, C .

BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (01) :81-87

[14] CATALYTIC ACTIVITY AND DENATURATION OF ENZYMES IN WATER ORGANIC COSOLVENT MIXTURES - ALPHA-CHYMOTRYPSIN AND LACCASE IN MIXED WATER ALCOHOL, WATER GLYCOL AND WATER FORMAMIDE SOLVENTS [J].

MOZHAEV, VV ;

KHMELNITSKY, YL ;

SERGEEVA, MV ;

BELOVA, AB ;

KLYACHKO, NL ;

LEVASHOV, AV ;

MARTINEK, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 184 (03) :597-602

[15] PERMEABILIZATION OF ESCHERICHIA-COLI-CELLS FOR ENHANCED PENICILLIN ACYLASE ACTIVITY [J].

NAGALAKSHMI, V ;

PAI, JS .

BIOTECHNOLOGY TECHNIQUES, 1994, 8 (06) :431-434

[16] Immobilization of whole cell penicillin G acylase in open pore gelatin matrix [J].

Norouzian, D ;

Javadpour, S ;

Moazami, N ;

Akbarzadeh, A .

ENZYME AND MICROBIAL TECHNOLOGY, 2002, 30 (01) :26-29

[17] RECOMBINANT WHOLE-CELL PENICILLIN ACYLASE BIOCATALYST - PRODUCTION, CHARACTERIZATION AND USE IN THE SYNTHESIS AND HYDROLYSIS OF ANTIBIOTICS [J].

OSPINA, S ;

MERINO, E ;

RAMIREZ, OT ;

LOPEZMUNGUIA, A .

BIOTECHNOLOGY LETTERS, 1995, 17 (06) :615-620

[18] ELECTROSTATIC EFFECTS IN PROTEINS [J].

PERUTZ, MF .

SCIENCE, 1978, 201 (4362) :1187-1191

[19] IMMOBILIZATION OF PERMEABILIZED ESCHERICHIA-COLI-CELLS WITH PENICILLIN ACYLASE ACTIVITY [J].

PRABHUNE, AA ;

RAO, BS ;

PUNDLE, AV ;

SIVARAMAN, H .

ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (02) :161-163

[20] KINETIC-STUDY OF PENICILLIN ACYLASE PRODUCTION BY RECOMBINANT ESCHERICHIA-COLI IN BATCH CULTURES [J].

RAMIREZ, OT ;

ZAMORA, R ;

ESPINOSA, G ;

MERINO, E ;

BOLIVAR, F ;

QUINTERO, R .

PROCESS BIOCHEMISTRY, 1994, 29 (03) :197-206