Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana

被引：178

作者：

Ishikawa, F

Suga, S

Uemura, T

Sato, MH

Maeshima, M ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Lab Cell Dynam, Nagoya, Aichi 4648601, Japan

[2] Kyoto Univ, Grad Sch Human & Environm Studies, Kyoto 6068501, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 25期

关键词：

aquaporin; ER; small and basic intrinsic protein; water channel; Arabidopsis;

D O I：

10.1016/j.febslet.2005.09.076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-beta-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions. (c) 2005 Published by Elsevier B.V.

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页码：5814 / 5820

页数：7

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