A simple method for constructing microinjectrodes for reversible inactivation in behaving monkeys

被引:32
作者
Chen, LTL [1 ]
Goffart, L
Sparks, DL
机构
[1] Baylor Coll Med, Div Neurosci, Houston, TX 77030 USA
[2] INSERM, U534, F-69500 Bron, France
关键词
injection-microelectrode; reversible inactivation; head-unrestrained; cortex; monkey;
D O I
10.1016/S0165-0270(01)00354-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for constructing a simple, durable injection-microelectrode (injectrode) is described. The injectrode can record neuronal activity, stimulate neuronal tissues, or inject substances locally through its tip. The injectrode is lightweight and is easy to construct from commercially available parts, and it can be used repeatedly for multiple recordings and injections. Since dura penetration can damage fragile electrode tips, a reliable method to pass the injectrode through an intact dura matter is described. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:81 / 85
页数:5
相关论文
共 12 条
[1]   A GRID SYSTEM AND A MICROSYRINGE FOR SINGLE CELL RECORDING [J].
CRIST, CF ;
YAMASAKI, DSG ;
KOMATSU, H ;
WURTZ, RH .
JOURNAL OF NEUROSCIENCE METHODS, 1988, 26 (02) :117-122
[2]   Acute activation and inactivation of macaque frontal eye field with GABA-related drugs [J].
Dias, EC ;
Kiesau, M ;
Segraves, MA .
JOURNAL OF NEUROPHYSIOLOGY, 1995, 74 (06) :2744-2748
[3]   A pressure system for the microinjection of substances into the brain of awake monkeys [J].
Dias, EC ;
Segraves, MA .
JOURNAL OF NEUROSCIENCE METHODS, 1997, 72 (01) :43-47
[4]  
EVERTS EV, 1966, METHOD MED RES, V11, P241
[5]  
Goffart Laurent, 1999, Society for Neuroscience Abstracts, V25, P5
[6]   Spatial and temporal parameters of cortical inactivation by GABA [J].
Hupé, JM ;
Chouvet, G ;
Bullier, J .
JOURNAL OF NEUROSCIENCE METHODS, 1999, 86 (02) :129-143
[7]   POPULATION CODING OF SACCADIC EYE-MOVEMENTS BY NEURONS IN THE SUPERIOR COLLICULUS [J].
LEE, CK ;
ROHRER, WH ;
SPARKS, DL .
NATURE, 1988, 332 (6162) :357-360
[8]  
Lemon R., 1984, METHODS NEURONAL REC
[9]   Reversible inactivation of subcortical sites by drug injection [J].
Malpeli, JG .
JOURNAL OF NEUROSCIENCE METHODS, 1999, 86 (02) :119-128
[10]   METHOD OF REVERSIBLE INACTIVATION OF SMALL REGIONS OF BRAIN-TISSUE [J].
MALPELI, JG ;
SCHILLER, PH .
JOURNAL OF NEUROSCIENCE METHODS, 1979, 1 (02) :143-151