Microfluidic Channels Fabricated on Mesoporous Electrospun Fiber Mats: A Facile Route to Microfluidic Chips

被引:17
作者
Jo, Eunmin [3 ]
Lim, Min-Cheol [1 ,2 ]
Kim, Han-Nah [1 ,2 ]
Paik, Hyun-Jong [4 ]
Kim, Young-Rok [1 ,2 ]
Jeong, Unyong [3 ]
机构
[1] Kyung Hee Univ, Coll Life Sci, Inst Life Sci & Resources, Yongin, South Korea
[2] Kyung Hee Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Yongin, South Korea
[3] Yonsei Univ, Dept Mat Sci & Engn, Seoul 120749, South Korea
[4] Pusan Natl Univ, Dept Polymer Sci & Engn, Pusan, South Korea
关键词
blends; biological applications of polymers; electrospinning; electrospun fibers; fibers; microfluidic channels; mesoporous fibers; protein chips; DESIGN; PURIFICATION; NANOFIBERS; PROTEINS; SURFACES;
D O I
10.1002/polb.22147
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
We report a facile way of preparing microfluidic channels filled with electrospun functional fibers. Patterned elastic molds were in tight contact with electrospun fiber mats without any leak of the analyte solution. As an example of the simple devices, we demonstrated a microfluidic protein chip selectively purifying histidine-tagged proteins. Highly mesoporous nitrilotriacetic acid-functionalized polystyrene (PS-NTA) fibers were produced by taking advantage of interpenetrating phase separation between PS and PEO during electrospinning. The specific interaction of Ni-complexed PS-NTA fibers with histidine enabled us to immobilize only target proteins from highly heterogeneous protein mixtures. The easy process to fabricate functionalized microchannels combined with the high production throughput from electrospinning may greatly contribute to chip-based chromatographic and bioanalytical devices. (C) 2010 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 49: 89-95, 2011
引用
收藏
页码:89 / 95
页数:7
相关论文
共 23 条
[1]   Patterned delivery of immunoglobulins to surfaces using microfluidic networks [J].
Delamarche, E ;
Bernard, A ;
Schmid, H ;
Michel, B ;
Biebuyck, H .
SCIENCE, 1997, 276 (5313) :779-781
[2]   Fiber-packed channel bioreactor for microfluidic protein digestion [J].
Fan, Huizhi ;
Chen, Gang .
PROTEOMICS, 2007, 7 (19) :3445-3449
[3]   METHODS AND REAGENTS - PURIFICATION OF HIS-TAG FUSION PROTEINS FROM ESCHERICHIA-COLI [J].
HENGEN, PN .
TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (07) :285-286
[4]   GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT [J].
HOCHULI, E ;
BANNWARTH, W ;
DOBELI, H ;
GENTZ, R ;
STUBER, D .
BIO-TECHNOLOGY, 1988, 6 (11) :1321-1325
[5]   Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend [J].
Kenawy, ER ;
Bowlin, GL ;
Mansfield, K ;
Layman, J ;
Simpson, DG ;
Sanders, EH ;
Wnek, GE .
JOURNAL OF CONTROLLED RELEASE, 2002, 81 (1-2) :57-64
[6]   Cell research with physically modified microfluidic channels: A review [J].
Kim, Sun Min ;
Lee, Sung Hoon ;
Suh, Kahp Yang .
LAB ON A CHIP, 2008, 8 (07) :1015-1023
[7]   Electrospinning of nanofibers: Reinventing the wheel? [J].
Li, D ;
Xia, YN .
ADVANCED MATERIALS, 2004, 16 (14) :1151-1170
[8]   Using liquid crystals to amplify protein-receptor interactions: Design of surfaces with nanometer-scale topography that present histidine-tagged protein receptors [J].
Luk, YY ;
Tingey, ML ;
Hall, DJ ;
Israel, BA ;
Murphy, CJ ;
Bertics, PJ ;
Abbott, NL .
LANGMUIR, 2003, 19 (05) :1671-1680
[9]  
Ma PX, 1999, J BIOMED MATER RES, V46, P60, DOI 10.1002/(SICI)1097-4636(199907)46:1<60::AID-JBM7>3.0.CO
[10]  
2-H